A concept for a pure DNA hydrogel has been demonstrated in which triplex structures act as the core components and are assisted by single strands and artfully designed Y-shaped structures. Typically, the triplex structure which is based on protonated cytosine–guanine–cytosine (C–G·C+) is formed at pH 5.0 and disassembles at pH 7.0, whereas the thymine–adenine–thymine (T–A·T)-based triplex structure is formed at pH 7.0 and disassembles at pH 10.0. Therefore, such triplex DNA structure-based pure DNA hydrogels provide pH-controlled reversible self-assembly of DNA structures with a transition between gel and liquid states. More significantly, the introduction of both a fluorophore (FAM) and a quencher (BHQ1) to the hydrogels provides an innovative method for monitoring the self-assembly and disassembly processes through a fluorescence technology.

中文翻译：

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pH控制的双向纯净DNA水凝胶：可逆的自组装和荧光监测†

已经证明了纯DNA水凝胶的概念，其中三链体结构充当核心成分，并由单链和巧妙设计的Y形结构辅助。通常，基于质子化胞嘧啶-鸟嘌呤-胞嘧啶（C-G·C +）的三链体结构在pH 5.0时形成并在pH 7.0时分解，而基于胸腺嘧啶-腺嘌呤-胸腺嘧啶（T-A·T）的三链体结构在pH 7.0时会形成结构，在pH 10.0时会分解。因此，这种基于三链DNA结构的纯DNA水凝胶提供了pH控制的DNA结构的可逆自组装，并在凝胶和液态之间转变。更重要的是，将荧光团（FAM）和淬灭剂（BHQ1）引入水凝胶为通过荧光技术监测自组装和拆卸过程提供了一种创新方法。