Bioelectrochemistry ( IF 4.8 ) Pub Date : 2018-04-09 Mohamad Farid Abd Muain, Kooi Hoong Cheo, Muhamad Nadzmi Omar, Amir Syahir Amir Hamzah, Hong Ngee Lim, Abu Bakar Salleh, Wen Siang Tan, Asilah Ahmad Tajudin
Hepatitis B virus core antigen (HBcAg) is the major structural protein of hepatitis B virus (HBV). The presence of anti-HBcAg antibody in a blood serum indicates that a person has been exposed to HBV. This study demonstrated that the immobilization of HBcAg onto the gold nanoparticles-decorated reduced graphene oxide (rGO-en-AuNPs) nanocomposite could be used as an antigen-functionalized surface to sense the presence of anti-HBcAg. The modified rGO-en-AuNPs/HBcAg was then allowed to undergo impedimetric detection of anti-HBcAg with anti-estradiol antibody and bovine serum albumin as the interferences. Upon successful detection of anti-HBcAg in spiked buffer samples, impedimetric detection of the antibody was then further carried out in spiked human serum samples. The electrochemical response showed a linear relationship between electron transfer resistance and the concentration of anti-HBcAg ranging from 3.91 ng mL−1 to 125.00 ng mL−1 with lowest limit of detection (LOD) of 3.80 ng mL−1 at 3 σ m−1. This established method exhibits potential as a fast and convenient way to detect anti-HBcAg.
中文翻译:
纳米金修饰的氧化石墨烯还原抗乙肝病毒核心抗原
乙型肝炎病毒核心抗原(HBcAg)是乙型肝炎病毒(HBV)的主要结构蛋白。血清中存在抗HBcAg抗体表明一个人已暴露于HBV。这项研究表明,将HBcAg固定在金纳米颗粒装饰的还原氧化石墨烯(rGO-en-AuNPs)纳米复合材料上可以用作抗原功能化的表面,以检测抗HBcAg的存在。然后,将修饰的rGO-en-AuNPs / HBcAg用抗雌二醇抗体和牛血清白蛋白作为干扰物进行抗-HBcAg的阻抗测定。在加标的缓冲液样品中成功检测到抗HBcAg后,然后在加标的人血清样品中进一步进行抗体的阻抗测定。-1至125.00纳克毫升-1与3.80检测(LOD)纳克毫升最低限度-1在3σ米-1。这种建立的方法具有潜力,可以作为一种快速便捷的检测抗HBcAg的方法。