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The mechanism and regularity of quenching the effect of bases on fluorophores: the base-quenched probe method
The Analyst Pub Date : 2018-04-09 , DOI: 10.1039/c8an00116b Huihui Mao 1, 2, 3, 4, 5 , Guanghua Luo 1, 2, 3, 4, 5 , Yuxia Zhan 1, 2, 3, 4, 5 , Jun Zhang 1, 2, 3, 4, 5 , Shuang Yao 1, 2, 3, 4, 5 , Yang Yu 1, 2, 3, 4, 5
中文翻译:
碱基对荧光团影响的猝灭机制和规律:碱基猝灭探针法
更新日期:2018-04-09
The Analyst Pub Date : 2018-04-09 , DOI: 10.1039/c8an00116b Huihui Mao 1, 2, 3, 4, 5 , Guanghua Luo 1, 2, 3, 4, 5 , Yuxia Zhan 1, 2, 3, 4, 5 , Jun Zhang 1, 2, 3, 4, 5 , Shuang Yao 1, 2, 3, 4, 5 , Yang Yu 1, 2, 3, 4, 5
Affiliation
The base-quenched probe method for detecting single nucleotide polymorphisms (SNPs) relies on real-time PCR and melting-curve analysis, which might require only one pair of primers and one probe.
中文翻译:
碱基对荧光团影响的猝灭机制和规律:碱基猝灭探针法
用于检测单核苷酸多态性 (SNP) 的碱基淬灭探针方法依赖于实时 PCR 和熔解曲线分析,可能只需要一对引物和一个探针。