Objective

To evaluate the in vitro cytotoxicity of Equia Forte (GC, Tokyo, Japan) and Ionostar Molar (Voco, Cuxhaven, Germany) on human dental pulp stem cells (hDPSCs).

Methods

hDPSCs isolated from third molars were exposed to several dilutions of Equia Forte and Ionostar Molar eluates (1/1, 1/2 and 1/4). These eluates were obtained by storing material samples in respective cell culture medium for 24 h (n = 40). hDPSCs in basal growth culture medium were the control. Cell viability and cell migration assays were performed using the MTT and wound-healing assays, respectively. Also, induction of apoptosis and changes in cell phenotype were evaluated by flow cytometry. Changes in cell morphology were analysed by immunocytofluorescence staining. To evaluate cell attachment to the different materials, hDPSCs were directly seeded onto the material surfaces and analyzed by scanning electron microscopy (SEM). The chemical composition of the materials was determined by energy dispersive X-ray (EDX) and eluates were analyzed by inductively coupled plasma-mass spectrometry (ICP-MS). Statistical analysis was performed with analysis of variance (ANOVA) and Student’s t-test (α < 0.05).

Results

Undiluted Equia Forte extracts led to a similar cell proliferation rates than the control group from 72 h onwards. There were no significance differences between Equia Forte and Ionostar Molar in terms of cell apoptosis and phenotype. However, in presence of Equia extracts the migration capacity of hDPSCs was higher than in presence of Ionostar Molar (p < 0.05). Also, SEM studies showed a higher degree of cell attachment when Equia Forte extracts were used. Finally, EDX analysis pointed to different weight percentages of C, O and Ca ions in glass ionomer cements, while other elements such as La, Al, Si, W, Mo and F were also detected.

Significance

In summary, Equia Forte promoted better biological responses in hDPSCs than Ionostar Molar.

中文翻译：

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热固性玻璃离子聚合物可促进人类牙髓干细胞的多种生物学反应

客观的

为了评估Equita Forte（GC，日本东京）和Ionostar Molar（Voco，Cuxhaven，德国）对人牙髓干细胞（h DPSCs）的体外细胞毒性。

方法

ħ从第三磨牙中分离牙髓干细胞暴露于Equia复地和Ionostar摩尔洗脱液（1/1，1/2和1/4）的几个稀释度。通过将材料样品在各自的细胞培养基中保存24小时（n = 40）来获得这些洗脱液。h在基础生长培养基中的DPSCs为对照。分别使用MTT和伤口愈合分析进行细胞活力和细胞迁移分析。另外，通过流式细胞术评估凋亡的诱导和细胞表型的变化。通过免疫细胞荧光染色分析细胞形态的变化。要评估细胞对不同材料的附着力，h将DPSC直接接种到材料表面上，并通过扫描电子显微镜（SEM）进行分析。通过能量色散X射线（EDX）确定材料的化学成分，并通过电感耦合等离子体质谱（ICP-MS）分析洗脱液。使用方差分析（ANOVA）和学生t检验（α  <0.05）进行统计分析。

结果

从72小时开始，未稀释的Equia Forte提取物导致的细胞增殖速率与对照组相似。在细胞凋亡和表型方面，Equia Forte和Ionostar Molar之间没有显着差异。但是，在有Equia提取物的情况下，h DPSC的迁移能力要比在Ionostar磨牙的情况下高（p  <  0.05）。此外，SEM研究显示，当使用Equia Forte提取物时，细胞附着的程度更高。最后，EDX分析指出玻璃离聚物水泥中C，O和Ca离子的重量百分比不同，同时还检测到其他元素，例如La，Al，Si，W，Mo和F。

意义

总而言之，Equia Forte在h DPSC中促进了比Ionostar Molar更好的生物学反应。