A green, highly sensitive and selective electrochemical imprinted biosensor for horseradish peroxidase (HRP) was designed and prepared based on the combination of polyaniline nanotubes (PANTs) and self-assembly surface molecular imprinting technique. PANI nanotubes, as supporting material, could effectively enhance imprinting efficiency and the electrode conductivity and facilitate electron transfer. The imprinted biosensor was characterized by electrochemical methods, Fourier transform infrared spectroscopy (FTIR) and scanning electron microscope (SEM). It showed a very highly electrocatalytical activity for reduction of hydrogen peroxide and could be used in amperometric determination of H₂O₂ in the ranges of 0–10 μM and 10–90 μM with a detection limit of 0.01 μM (S / N = 3). The imprinted sensor showed still an excellent selectivity for HRP and was successfully employed to detect different concentration levels of HRP from 1.0 × 10⁻⁹ to 0.10 mg/mL with a detection limit of 3.56 × 10⁻¹⁰ mg/mL(8.1fM) (S / N = 3) with Fe(CN)₆^3/4− probe by differential pulse voltammetry(DPV). The developed method was successfully used in determination of H₂O₂ in human urine sample.

基于聚苯胺纳米管（PANTs）和自组装表面分子印迹技术的结合，设计并制备了一种绿色，高度灵敏，选择性强的辣根过氧化物酶（HRP）电化学印迹生物传感器。作为支撑材料的PANI纳米管可以有效地提高压印效率和电极电导率，并促进电子转移。印迹生物传感器通过电化学方法，傅立叶变换红外光谱（FTIR）和扫描电子显微镜（SEM）进行了表征。它对还原过氧化氢显示出非常高的电催化活性，可用于安培法测定H ₂ O _2。检出限为0.01μM（S / N = 3），范围为0-10μM和10-90μM。印迹传感器对HRP仍显示出极好的选择性，并成功用于检测HRP浓度从1.0×10 ^-9到0.10 mg / mL的不同浓度水平，检出限为3.56×10 ^-10  mg / mL（8.1fM）（ S / N = 3）以Fe（CN）₆ ^{3/4 -}探针由微分脉冲伏安法（DPV）。该方法成功用于人尿样中H ₂ O _2的测定。