Nerve conduits are essential for guiding the regeneration of injured peripheral nerves, and empty conduits usually cannot perform satisfactorily due to problems with cell migration and nutrient transportation. Taking the electrophysiological features of nervous tissues into consideration, in this study, a conductive conduit filled with parallel-aligned conductive fibers was constructed, and its potential for enhancing peripheral nerve regeneration was evaluated in vivo. The conductive fibers were prepared via depositing a polypyrrole (PPY) shell onto electrospun parallel-aligned poly(lactide-co-glycolide) (PLGA) fibers. The conductive conduit was prepared from PLGA/PPY emulsion via dip-coating on a mandrel. Both materials were non-cytotoxic to PC12 cells and were able to promote cell proliferation and differentiation. Moreover, the aligned fibers provided strong orientation guidance for nerve fibers. Sciatic nerve defects were created in Sprague-Dawley rats, and empty or fiber-filled conduits were sutured into the defects. Meanwhile, the control groups received PLGA conduits or autografts. Twelve weeks post-operation, the fiber-filled conductive conduit showed much better nerve regeneration outcomes than both the PLGA conduit and the empty conductive conduit and showed comparable results to the autograft in terms of electrophysiological properties, sciatic function indices, and regenerated myelinated nerve fibers as well as axon diameter and myelin thickness. It is possible that the oriented conductive fibers in the conductive conduit provide a favorable micro-environment for nerve growth due to their capacity to transmit self-originated electrical stimulation between cells. The results of animal testing confirmed the feasibility of using combined conductive conduits for guiding nerve regeneration.

神经导管对于引导受伤的周围神经再生至关重要，而空导管通常由于细胞迁移和营养运输方面的问题而不能令人满意地发挥作用。考虑到神经组织的电生理特征，在本研究中，构建了填充有平行排列的导电纤维的导电导管，并在体内评估了其增强周围神经再生的潜力。。通过将聚吡咯（PPY）壳沉积到电纺平行排列的聚（丙交酯-乙交酯）（PLGA）纤维上来制备导电纤维。导电导管是由PLGA / PPY乳液通过浸涂在心轴上制成的。两种材料均对PC12细胞无细胞毒性，并能够促进细胞增殖和分化。而且，排列的纤维为神经纤维提供了强有力的定向引导。在Sprague-Dawley大鼠中产生坐骨神经缺损，并将空的或充满纤维的导管缝合到缺损中。同时，对照组接受PLGA导管或自体移植物。术后十二周，充满纤维的导电导管比PLGA导管和空导管显示出更好的神经再生结果，并且在电生理特性，坐骨神经功能指数，再生髓鞘神经纤维以及轴突直径和神经轴突直径方面表现出与自体移植相当的结果。髓磷脂的厚度。导电导管中的定向导电纤维由于其在细胞之间传递自发的电刺激的能力而可能为神经生长提供有利的微环境。动物试验的结果证实了使用组合的导电导管引导神经再生的可行性。导电导管中的定向导电纤维由于其在细胞之间传递自发的电刺激的能力而可能为神经生长提供有利的微环境。动物试验的结果证实了使用组合的导电导管引导神经再生的可行性。导电导管中的定向导电纤维由于其在细胞之间传递自发的电刺激的能力而可能为神经生长提供有利的微环境。动物试验的结果证实了使用组合的导电导管引导神经再生的可行性。