The aim of this study was to evaluate the enhancement of biogas production from cattle manure (CM) at pilot-plant scale using: a) ultrasound pretreatment (US) (520 kJ/kg Total Solids (TS); 409.59 kJ), b) co-digestion with crude glycerine (Gly) from the biodiesel industry (6% w/w), and c) US pretreatment (520 kJ/kg TS; 340.84 kJ), applied to the aforementioned mixture of both substrates prior to anaerobic co-digestion. The reactor used for this purpose was an Induced Bed Reactor (IBR), with a useful volume of 1250 L. Methane production from CM was enhanced by the application of low-energy ultrasound pretreatment (520 kJ/kg TS) (from 0.29 to 0.46 m³ CH₄/kg Volatile Solids (VS_added)), and by co-digestion with Gly (from 0.29 to 0.44 m³ CH₄/kg VS_added). The best results were obtained when the mixture of CM + Gly was pretreated by US (up to 0.59 m³ CH₄/kg VS_added). Observation by Scanning Electron Microscopy (SEM) of the microstructural changes the CM underwent after pretreatment and/or co-digestion with crude glycerine enabled the discussion and justification of the results obtained in the present study.

这项研究的目的是使用以下方法评估中试规模牛粪（CM）沼气产量的提高：a）超声预处理（美国）（520 kJ / kg总固体（TS）； 409.59 kJ），b）与生物柴油行业的粗甘油（Gly）共消化（6％w / w），和c）美国预处理（520 kJ / kg TS; 340.84 kJ），在厌氧共处理之前应用于上述两种底物的混合物消化。为此目的使用的反应器是感应床反应器（IBR），有用容积为1250L。通过应用低能超声预处理（520 kJ / kg TS）（0.29至0.46）可以提高CM甲烷的生产量m ³ CH ₄ / kg挥发性固体（_添加VS ），并与Gly共消化（0.29至0.44 m ³CH ₄ / kg V​​S_添加）。当CM +甘氨酸的混合物通过美国预处理（最多0.59米获得了最好的结果³ CH ₄ / kg的VS_添加）。通过扫描电子显微镜（SEM）观察，对CM进行预处理和/或与粗甘油共消化后所经历的CM的微观结构变化，使得能够对本研究中获得的结果进行讨论和论证。