Scattering and absorption limit the penetration of optical fields into tissue. We demonstrate a new approach for increased depth penetration in light-sheet microscopy: attenuation-compensation of the light field. This tailors an exponential intensity increase along the illuminating propagation-invariant field, enabling the redistribution of intensity strategically within a sample to maximize signal and minimize irradiation. A key attribute of this method is that only minimal knowledge of the specimen transmission properties is required. We numerically quantify the imaging capabilities of attenuation-compensated Airy and Bessel light sheets, showing that increased depth penetration is gained without compromising any other beam attributes. This powerful yet straightforward concept, combined with the self-healing properties of the propagation-invariant field, improves the contrast-to-noise ratio of light-sheet microscopy up to eightfold across the entire field of view in thick biological specimens. This improvement can significantly increase the imaging capabilities of light-sheet microscopy techniques using Airy, Bessel, and other propagation-invariant beam types, paving the way for widespread uptake by the biomedical community.

中文翻译：

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具有衰减补偿的传播不变光束的光片显微镜。

散射和吸收限制了光场进入组织的渗透。我们展示了一种在光片显微镜中增加深度穿透的新方法：光场的衰减补偿。这样可以沿着照度不变的传播区域调整指数强度的增加，从而有策略地在样本内重新分配强度，以使信号最大化并使辐射最小化。该方法的一个关键特性是，仅需要对标本传输特性的最少了解。我们在数值上量化了衰减补偿的艾里和贝塞尔光片的成像能力，表明在不损害任何其他光束属性的情况下获得了增加的深度穿透力。这个强大而简单的概念 结合不变传播场的自愈特性，可将厚生物样本中整个光学视场的光片显微镜对比度提高至八倍。这种改进可以显着提高使用Airy，Bessel和其他传播恒定光束类型的光片显微镜技术的成像能力，从而为生物医学界的广泛采用铺平了道路。