AbstractWe propose a novel concept for cellular scaffolds with 2D-patterned mechanical properties. Thin films of glassy polystyrene (PS) with thicknesses ranging from 100 nm to 1 μm were prepared on epoxy resin-based line and space (L&S) patterned substrates. Although the outermost surface of PS on the L- and S-regions was sufficiently flat at the same level, the mechanical responses differed depending on the presence of the underlying resin foundation. The initial cell adhesion and spreading and the proliferation on the scaffolds were affected by the 2D-patterned mechanical properties, that is, cellular behavior was suppressed on mechanically unstable S-regions.A novel concept for cellular scaffolds with 2D-patterned mechanical properties was proposed. Thin films of glassy polystyrene (PS) with thicknesses ranging from 100 nm to 1 μm were prepared on epoxy resin-based line and space (L&S) patterned substrates. Although the outermost surface of PS was sufficiently flat regardless of the L&S patterned substrates, the mechanical responses differed depending on the presence of the underlying resin foundation. The initial cell adhesion and spreading and the proliferation on the scaffolds were affected by the 2D-patterned mechanical properties.

中文翻译：

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具有二维图案机械性能的聚合物支架上的细胞行为

摘要我们提出了一种具有二维图案机械性能的细胞支架的新概念。在基于环氧树脂的线和空间 (L&S) 图案基板上制备厚度范围为 100 nm 至 1 μm 的玻璃态聚苯乙烯 (PS) 薄膜。尽管 L 和 S 区域上 PS 的最外表面在同一水平上足够平坦，但机械响应因下面树脂基础的存在而异。支架上的初始细胞粘附和扩散以及增殖受 2D 图案机械性能的影响，即细胞行为在机械不稳定的 S 区受到抑制。提出了具有 2D 图案机械性能的细胞支架的新概念. 在基于环氧树脂的线和空间 (L&S) 图案基板上制备厚度范围为 100 nm 至 1 μm 的玻璃态聚苯乙烯 (PS) 薄膜。尽管无论 L&S 图案基板如何，PS 的最外表面都足够平坦，但机械响应因下面树脂基础的存在而异。支架上的初始细胞粘附和扩散以及增殖受 2D 图案机械性能的影响。