In this study, biomass-hydrolysate was used for enhancing the bioreduction of ethyl 4-chloro-3-oxobutanoate (COBE). Firstly, dilute alkalic salt pretreatment was attempted to pretreat bamboo shoot shell (BSS). It was found that enzymatic in situ hydrolysis of 20-50 g/L BSS pretreated with dilute alkalic salts (0.4% Na₂CO₃, 0.032% Na₂S) at 7.5% sulfidity by autoclaving at 110 °C for 40 min gave sugar yields at 59.9%-73.5%. Moreover, linear relationships were corrected on solid recovery-total delignification-sugar yield. In BSS-hydrolysates, xylose and glucose could promote the reductase activity of recombinant E. coli CCZU-A13. Compared with glucose, hydrolysate could increase the reductase activity by 1.35-folds. Furthermore, the cyclohexane-hydrolysate (10:90, v/v) biphasic media containing ethylene diamine tetraacetic acid (EDTA,40 mM) and L-glutamine (150 mM) was built for the effective biosynthesis of ethyl (R)-4-chloro-3-hydroxybutanoate [(R)-CHBE] (94.6% yield) from 500 mM COBE. In conclusion, this strategy has high potential for the effective biosynthesis of (R)-CHBE (>99% e.e.).

在这项研究中，生物质水解产物用于增强4-氯-3-氧代丁酸乙酯（COBE）的生物还原。首先，尝试了稀碱盐预处理来预处理笋壳（BSS）。据发现，酶在原位的20-50克水解/ L BSS用稀碱性盐（0.4％的Na预处理₂ CO ₃，0.032％的Na ₂ S）在7.5％硫化通过在110℃下高压灭菌40分钟，得到糖收率为59.9％-73.5％。此外，对固体回收率-去木质素-糖总产量的线性关系进行了校正。在BSS水解产物中，木糖和葡萄糖可以促进重组大肠杆菌的还原酶活性。CCZU-A13。与葡萄糖相比，水解产物可将还原酶活性提高1.35倍。此外，构建了包含乙二胺四乙酸（EDTA，40 mM）和L-谷氨酰胺（150 mM）的环己烷水解产物（10:90，v / v）两相介质，以有效地生物合成乙基（R）-4-来自500 mM COBE的3-羟基羟丁酸氯[（R）-CHBE]（收率94.6％）。总之，该策略具有有效的生物合成（R）-CHBE（> 99％ee）的潜力。