Effects of bovine lactoferrin on l-DOPA absorption and metabolism in mice,Food & Function

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Effects of bovine lactoferrin on l-DOPA absorption and metabolism in mice
Food & Function ( IF 5.1 ) Pub Date : 2018-04-06 00:00:00 , DOI: 10.1039/c7fo01518f
Masayuki Ikeda _{1,

2,

3,

4,

5} , Hiroshi Iijima _{6,

7,

8,

9} , Ichizo Shinoda _{1,

2,

3,

4,

5} , Hiroshi Iwamoto _{1,

2,

3,

4,

5} , Yasuhiro Takeda _{1,

2,

3,

4,

5}

Affiliation

Bioactive natural products, habitually ingested with milk or its derivative nutrients, have been studied for their bioavailability. In this study, we investigated the effects of the co-administration of bovine milk-derived lactoferrin (bLF) and bioactive products, with a focus on catechol-O-methyltransferase (COMT), an enzyme in the catechol metabolism. bLF showed inhibitory activity on COMT in vitro, and acidic pretreatment of bLF enhanced its inhibitory activity. Moreover, partially digested products of bLF by pepsin retained inhibitory activity. Based on these results, bLF was co-administered with levodopa (L-DOPA), which is a catechol compound and a precursor of dopamine, and the effect of bLF on L-DOPA absorption and metabolism was investigated in a mouse model. The co-administration of L-DOPA and bLF alone showed no effect on the concentration of L-DOPA in plasma. However, with the additional administration of carbidopa, the concentration of L-DOPA was significantly enhanced. Furthermore, the ratio of L-DOPA/3-O-methyldopa significantly increased. On the other hand, casein, which is a major milk protein, was not effective. In addition, COMT activity in the intestines was lowered with bLF administration. We concluded that the co-administration of bLF and carbidopa enhances the concentration of L-DOPA.

中文翻译：

牛乳铁蛋白对小鼠1- DOPA吸收和代谢的影响

已经研究了习惯性地摄取牛奶或其衍生营养素的生物活性天然产物的生物利用度。在这项研究中，我们调查了牛乳来源的乳铁蛋白（bLF）和生物活性产品共同给药的效果，重点研究儿茶酚代谢中的一种酶儿茶酚-O-甲基转移酶（COMT）。bLF在体外对COMT表现出抑制活性，而bLF的酸性预处理增强了它的抑制活性。此外，胃蛋白酶部分消化的bLF产物保留了抑制活性。根据这些结果，bLF与左旋多巴（L -DOPA）（一种儿茶酚化合物和多巴胺的前体）共同给药，并且bLF对L-在小鼠模型中研究了DOPA的吸收和代谢。单独使用L -DOPA和bLF共同给药对血浆中L -DOPA的浓度没有影响。然而，通过额外施用卡比多巴，L -DOPA的浓度显着提高。此外，L -DOPA / 3- O-甲基多巴的比例显着增加。另一方面，酪蛋白是一种主要的乳蛋白，效果不佳。另外，bLF的施用降低了肠中的COMT活性。我们得出结论，bLF和卡比多巴的共同给药可提高L -DOPA的浓度。

更新日期：2018-04-06

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