A new strategy is developed for construction of the mixed molecular nanostructures from folic acid (FOA), a targeting agent, and deferrioxamoine-Ga(III), (DFO-Ga(III)), a theranostic agent, on gold-mercaptopropionic acid surface, Au-MPA. The strategy is focused to achieve a system in which all the active constituents of FOA; i.e., pteridine rings, p-aminobenzoeic acid, and the glutamic acid, having high affinity for folate receptor overexpressed on cancer cells; remain unreacted in adjacent to DFO-Ga(III), Au-MPA-[DFO-Ga(III)]‖-[FOA]. For this purpose, the NH₂ groups of FOA and DFO-Ga(III) were attached covalently and separately to COOH of Au-MPA surface allowing all the active groups of FOA to be available for drug delivery purposes. The data obtained through several electrochemical and surface analysis techniques, supported successful construction of the designed mixed molecular nanostructures system. In addition, the results showed that the system is stable, and Ga(III) ion does not leave DFO-Ga(III) complex. The prepared surface was successfully tested for capturing of the breast cancer cells 4 T1 as a model. The measurements showed a rapid uptake kinetics (t_1/2 of ~6.0 min) and efficient accessibility of the system by the cancer cells; the R_ct was significantly increased in the presence of 4 T1 cells compared with blank PBS (ΔR_ct ~420 kΩ).

开发了一种新的策略，用于在金巯基丙酸表面上从靶向剂叶酸（FOA）和治疗剂去铁氧黄嘌呤-Ga（III）（DFO-Ga（III））构造混合分子纳米结构。 ，金-MPA。该战略的重点是建立一个由FOA的所有活跃组成部分组成的系统；即，对在癌细胞上过表达的叶酸受体具有高亲和力的蝶啶环，对氨基苯甲酸和谷氨酸。在DFO-Ga（III），Au-MPA- [DFO-Ga（III）]′-[FOA]附近保持未反应。为此，将FOA和DFO-Ga（III）的NH ₂基团共价且分别地连接到Au-MPA表面的COOH，使FOA的所有活性基团均可用于药物输送。通过几种电化学和表面分析技术获得的数据支持成功设计了设计的混合分子纳米结构系统。此外，结果表明该系统是稳定的，并且Ga（III）离子不会离开DFO-Ga（III）络合物。成功地测试了制备的表面作为模型捕获的乳腺癌细胞4 T1的捕获。测量结果显示了快速的吸收动力学（〜6.0分钟的t _1/2）和癌细胞对系统的有效可达性。将R _CT在4个T1细胞的存在显著增加与空白相比，PBS（ΔR_克拉〜420千欧）。