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An insoluble iron complex coated cathode enhances direct electron uptake by Rhodopseudomonas palustris TIE-1
Bioelectrochemistry ( IF 4.8 ) Pub Date : 2018-04-05
Karthikeyan Rengasamy, Tahina Ranaivoarisoa, Rajesh Singh, Arpita Bose

Microbial electrosynthesis (MES) is a promising bioelectrochemical approach to produce biochemicals. A previous study showed that Rhodopseudomonas palustris TIE-1 can directly use poised electrodes as electron donors for photoautotrophic growth at cathodic potentials that avoid electrolytic H2 production (photoelectroautotrophy). To make TIE-1 an effective biocatalyst for MES, we need to improve its electron uptake ability and growth under photoelectroautotrophic conditions. Because TIE-1 interacts with various forms of iron while using it as a source of electrons for photoautotrophy (photoferroautotrophy), we tested the ability of iron-based redox mediators to enhance direct electron uptake. Our data show that soluble iron cannot act as a redox mediator for electron uptake by TIE-1 from a cathode poised at +100 mV vs. Standard Hydrogen electrode. We then tested whether an immobilized iron-based redox mediator Prussian blue (PB) can enhance electron uptake by TIE-1. Chronoamperometry indicates that cathodic current uptake by TIE-1 increased from 1.47 ± 0.04 to 5.6 ± 0.09 μA/cm2 (3.8 times). Overall, our data show that immobilized PB can enhance direct electron uptake by TIE-1.



中文翻译:

不溶性铁络合物包覆的阴极增强了红假单胞菌TIE-1的直接电子吸收

微生物电合成(MES)是一种有前途的生物电化学方法,用于生产生物化学物质。先前的研究表明,红假单胞菌TIE-1可以直接使用平衡的电极作为电子供体,以在避免电解H 2的阴极电势下进行光自养生长生产(光电自养)。为了使TIE-1成为MES的有效生物催化剂,我们需要提高其在光电子自养条件下的电子吸收能力和生长。由于TIE-1与多种形式的铁发生相互作用,同时将其用作光致自养(光铁自养)的电子源,因此我们测试了铁基氧化还原介体增强直接电子吸收的能力。我们的数据表明,与标准氢电极相比,可溶性铁不能充当TIE-1从+100 mV平衡的阴极吸收电子的氧化还原介体。然后,我们测试了固定化的铁基氧化还原介体普鲁士蓝(PB)是否可以增强TIE-1的电子吸收。计时电流分析法表明,TIE-1吸收的阴极电流从1.47±0.04增加到5.6±0.09μA/ cm 2(3.8倍)。总体而言,我们的数据表明,固定的PB可以增强TIE-1的直接电子吸收。

更新日期:2018-04-06
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