Widespread antibiotic use and the antimicrobial resistance phenomenon demand new analytical methods and the use of non-conventional matrices increasingly necessary for safe food control. We present a method developed to detect six common antibiotics used in poultry breeding, in the unconventional matrix, feathers, compared to muscle and liver. The analysis for the presence of two β-lactams (penicillin V, amoxicillin), two fluoroquinolones (enrofloxacin, ciprofloxacin), one phenicol (thiamphenicol) and one macrolide (tylosin) was validated and achieved by HPLC–HRMS, with the ultimate aim to identify untargeted metabolites in broilers subjected to different therapeutic protocols. All the validated method parameters met the regulatory requirements. Muscle and liver were not effective matrices when the withdrawal periods were largely respected. Conversely, feathers proved a promising matrix for the detection of all the studied antibiotics, in the range of 8.72-1885.32 ng g^-1, except penicillin V. Like other nonconventional matrices, such as teeth, the antibiotics detected in feathers existed in their unmetabolised form.

抗生素的广泛使用和抗药性现象需要新的分析方法，并且对于安全食品控制而言，非常规基质的使用日益必要。我们提出了一种方法，用于检测与肌肉和肝脏相比在家禽育种中使用的六种常见抗生素，包括非常规基质，羽毛。通过HPLC-HRMS验证并实现了对两种β-内酰胺（青霉素V，阿莫西林），两种氟喹诺酮类（恩诺沙星，环丙沙星），一种苯酚（噻吩甲酚）和一种大环内酯（泰乐菌素）的存在的分析，其最终目的是鉴定接受不同治疗方案的肉鸡中未靶向的代谢物。所有经过验证的方法参数均符合法规要求。当停药期被广泛考虑时，肌肉和肝脏不是有效的基质。除青霉素V外，^-1。与其他非常规基质（如牙齿）一样，在羽毛中检测到的抗生素以其未代谢的形式存在。