Electron mediators have the ability to facilitate electron acceptance and donation, which can accelerate the electron transfer during the electrochemical process. Few research has been reported about electroactive polymer nanospheres as redox mediators for sensing to date. In this study, we synthesized a new electroactive ferrocene derivative polymer nanospheres (FPS) via a facile and self-assembly method. The obtained FPS was characterized by UV–Vis spectra, Fourier transform infrared spectroscopy, transmission electron microscopy and dynamic light scattering. Based on the electroactive property of FPS, a new electrochemical sensor for acetaminophen (AP) based on the nanocomposite of multi-walled carbon nanotubes-ploy-diallyl-dimethyl-ammonium chloride-FPS (MWCNT- PDDA-FPS) was firstly developed. The greatly enhanced electrochemical performance towards AP determination can be obtained owing to the electrocatalysis of electroactive FPS and good conductivity of MWCNT. The AP sensor exhibits a wide sensing linear range from 3 to 1100 μM and the detection limit is 0.6 μM (S/N = 3). Therefore, these ferrocene derivative polymer nanospheres may become a new nanomaterial to construct of platforms for bioanalytical and electrochemical immune research in the future.

电子介体具有促进电子接受和捐赠的能力，这可以加速电化学过程中的电子转移。迄今为止，几乎没有关于电活性聚合物纳米球作为氧化还原介体进行传感研究的报道。在这项研究中，我们通过一种简便的自组装方法合成了一种新的电活性二茂铁衍生物聚合物纳米球（FPS）。所获得的FPS通过UV-Vis光谱，傅立叶变换红外光谱，透射电子显微镜和动态光散射进行了表征。基于FPS的电活性，首先开发了一种新型的对乙酰氨基酚（AP）电化学传感器，该传感器基于多壁碳纳米管-聚-二烯丙基-二甲基-氯化铵-FPS（MWCNT-PDDA-FPS）的纳米复合材料。由于电活性FPS的电催化作用和MWCNT的良好电导率，可以大大提高AP测定的电化学性能。AP传感器具有从3到1100μM的宽检测线性范围，检测极限为0.6μM（S / N = 3）。因此，这些二茂铁衍生物聚合物纳米球可能会成为未来构建生物分析和电化学免疫研究平台的新型纳米材料。