Hydroxyapatite (HAP) is a form of naturally occurring calcium apatite present in bone and tooth enamel. It is an important biomaterial with diverse biomedical applications such as a surface coating for metallic orthopedic implants. Synthesized pristine HAP has poor mechanical properties, inferior wear resistance and has limits for directly used in bone tissue engineering applications. To address these limitations, we synthesized a suitable orthopedic implant hybrid material (M-HAP/PCL/GO) by using positively charged calcium ions of mineralized HAP (M-HAP) combined with negatively charged graphene oxide-Polycaprolactone (GO-PCL). The successfully synthesized M-HAP/PCL/GO composite was comprehensively characterized by Fourier-transform infrared spectroscopy (FTIR), powder X-ray diffraction (XRD) and field-emission scanning electron microscopy (FE-SEM). The micro-hardness technique was used to determine the mechanical strength of M-HAP (315±4Hv), M-HAP/GO (370±3Hv) and M-HAP/PCL/GO (455±5Hv). M-HAP/PCL/GO was also tested for its anti-bactericidal impact against Staphylococcus aureus and Escherichia coli. MG63 osteoblast cells cultured on the M-HAP/PCL/GO composite (10 mg/mL) coated sample, displayed outstanding viability after 3 and 5 days of incubation at pH 7.4, which indicated that the composite is suitable material for bone implants and induces the cell proliferation. It was also tested in vivo in Wistar rats and was observably beneficial bone formation within 28 days post-implant operation. These tests proved that the M-HAP/PCL/GO composite can be considered as a prospective candidate for future bone implant applications.

羟基磷灰石（HAP）是存在于骨骼和牙齿珐琅质中的天然存在的磷灰石钙的一种形式。它是一种重要的生物材料，具有多种生物医学应用，例如用于金属骨科植入物的表面涂层。合成的原始HAP具有较差的机械性能，较差的耐磨性，并且直接在骨组织工程应用中具有局限性。为了解决这些局限性，我们通过使用带正电的矿化HAP的钙离子（M-HAP）与带负电的氧化石墨烯-聚己内酯（GO-PCL）合成了合适的整形外科植入物混合材料（M-HAP / PCL / GO）。通过傅立叶变换红外光谱（FTIR）对成功合成的M-HAP / PCL / GO复合材料进行了全面表征，粉末X射线衍射（XRD）和场发射扫描电子显微镜（FE-SEM）。使用显微硬度技术确定M-HAP（315±4Hv），M-HAP / GO（370±3Hv）和M-HAP / PCL / GO（455±5Hv）的机械强度。还对M-HAP / PCL / GO的抗细菌作用进行了测试金黄色葡萄球菌和大肠杆菌。在M-HAP / PCL / GO复合材料（10 mg / mL）包被的样品上培养的MG63成骨细胞在pH 7.4下孵育3天和5天后表现出出色的生存能力，这表明该复合材料是适合于骨植入物并诱导其生长的材料。细胞增殖。它还在Wistar大鼠体内进行了测试，在植入手术后28天内可观察到有益的骨形成。这些测试证明，M-HAP / PCL / GO复合材料可被视为未来骨植入物应用的潜在候选者。