Acrylamide is neurotoxic, genotoxic, teratogenic and carcinogenic. Its widespread use in various industrial processes leads to environmental contamination. Acrylamidase produced by certain bacteria degrade acrylamide to acrylic acid and ammonia. The present study details the isolation and identification of soil bacterium which could degrade acrylamide. Among the 18 acrylamide-degrading isolates tested, isolate ICTDB921 demonstrated superior acrylamide degradation which was confirmed by HPLC, FTIR and GC-MS. The partial 16S rRNA sequencing confirmed the isolate to be Cupriavidus oxalaticus ICTDB921, which showed highest growth at 60 mM acrylamide, neutral pH and 30 °C. The kinetic model predictions were consistent with experimental results. The acrylamidase from this isolate showed potency at pH (6–8) and temperatures (30–60 °C), with reasonable pH (6–8) and thermal stability (upto 60 °C). The enzyme was stable against most metal ions and amino acids, and also degraded other aliphatic amides, demonstrating its potential in remediation of acrylamide from the environment and food systems.

丙烯酰胺具有神经毒性，遗传毒性，致畸性和致癌性。它在各种工业过程中的广泛使用导致环境污染。某些细菌产生的丙烯酰胺酶将丙烯酰胺降解为丙烯酸和氨。本研究详细介绍了可降解丙烯酰胺的土壤细菌的分离和鉴定。在所测试的18种降解丙烯酰胺的菌株中，ICTDB921菌株表现出优异的丙烯酰胺降解能力，这已通过HPLC，FTIR和GC-MS证实。部分16S rRNA测序确认分离物为草酸铜杯ICTDB921，在60 mM丙烯酰胺，中性pH和30°C下显示出最高的增长。动力学模型的预测与实验结果一致。该分离株的丙烯酰胺酶在pH（6-8）和温度（30-60°C）下显示出效力，并具有合理的pH（6-8）和热稳定性（最高60°C）。该酶对大多数金属离子和氨基酸稳定，并且还降解了其他脂族酰胺，证明了其从环境和食品系统中修复丙烯酰胺的潜力。