This study investigated the greenhouse gas emission characteristics and microbial community dynamics with the variation of temperature during partial nitrification. Low temperature weakened nitrite accumulation, and partial nitrification would shift to complete nitrification easily at 15 °C. Based on CO₂ equivalents (CO₂-eq), partial nitrification process released 2.7 g of greenhouse gases per gMLSS per cycle, and N₂O accounted for more than 98% of the total CO₂-eq emission. The total CO₂-eq emission amount at 35 °C was 45.6% and 153.4% higher than that at 25 °C and 15 °C, respectively. During partial nitrification, the microbial community diversity greatly declined compared with seed sludge. However, the diversity was enhanced at low temperature. The abundance of Betaproteobacteria at class level increased greatly during partial nitrification. Proteobacteria abundance declined while Nitrospira raised at low temperature. The nosZ community abundance was not affected by temperature, although N₂O emission was varied with the operating temperature.

本研究调查了部分硝化过程中温室气体的排放特征和微生物群落动态随温度的变化。低温会削弱亚硝酸盐的积累，在15°C时，部分硝化作用会转变为完全硝化作用。基于CO ₂当量（CO ₂当量），部分硝化过程每个循环每gMLSS释放2.7 g温室气体，N ₂ O占总CO ₂当量排放的98％以上。总CO ₂35°C时的当量排放量分别比25°C和15°C时的排放量高45.6％和153.4％。与种子污泥相比，在部分硝化过程中，微生物群落多样性大大降低。然而，在低温下多样性得到增强。在部分硝化过程中，班级水平β变形杆菌的丰度大大增加。低温下硝化螺菌升高时，细菌变形杆菌的丰度下降。所述nosZ社区丰度不受温度影响，作为n ₂ O排放用的操作温度变化。