Supported ionic liquid phase (SILP) facilitated gas-phase enzyme catalysis – CALB catalyzed transesterification of vinyl propionate†,Catalysis Science & Technology

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Supported ionic liquid phase (SILP) facilitated gas-phase enzyme catalysis – CALB catalyzed transesterification of vinyl propionate†
Catalysis Science & Technology ( IF 4.4 ) Pub Date : 2018-04-05 00:00:00 , DOI: 10.1039/c8cy00089a
Changhee Lee _{1,

2,

3,

4} , Bernhard Sandig _{4,

5,

6,

7} , Michael R. Buchmeiser _{4,

5,

6,

7} , Marco Haumann _{1,

2,

3,

4}

Affiliation

The supported ionic liquid phase (SILP) technology has been used to immobilize Candida Antarctica Lipase B (CALB) within a hybrid monolith. The monolith was synthesized in situ inside a Teflon® cartridge via the 4-(dimethylamino)pyridine-catalyzed polyaddition of 1,1,1-tris(hydroxymethyl)propane to 4,4′-methylenebis(phenylisocyanate) in the presence of porous cellulose-2.5-acetate beads. An ionic liquid containing small amounts of CALB was impregnated into these beads and the resulting enzyme-SILP (e-SILP) catalyst was tested in the continuous gas-phase transesterification of vinyl propionate and 2-propanol. The enzyme was catalytically active with conversions exceeding 30% at short contact times of only 24 s. Total turn-over numbers of 1.7 × 10⁸ and 2.4 × 10⁸ mol_substrate mol_CALB⁻¹ could be obtained for the two e-SILP cartridges tested. High stability could be achieved at relatively high reaction temperatures around 65 °C for more than 700 h time on stream.

中文翻译：

支持的离子液相（SILP）促进了气相酶催化– CALB催化丙酸乙烯酯的酯交换反应†

受支持的离子液相（SILP）技术已用于将南极假丝酵母脂肪酶B（CALB）固定在杂交整料中。在多孔纤维素存在下，通过4-（二甲氨基）吡啶催化的1,1,1-三（羟甲基）丙烷与4,4'-亚甲基双（苯基异氰酸酯）的加成加成反应，在Teflon®滤芯内原位合成整料。-2.5-乙酸盐珠。将含有少量CALB的离子液体浸渍到这些珠粒中，并在丙酸乙烯酯和2-丙醇的连续气相酯交换反应中测试所得的酶-SILP（e-SILP）催化剂。该酶具有催化活性，在短短的24 s接触时间内转化率超过30％。总周转数1.7×10 ⁸对于两个测试的e-SILP滤芯，可以获得2.4×10 ⁸ mol_底物mol _CALB^-1。在相对较高的反应温度（约65°C）下运行700小时以上，可以实现高稳定性。

更新日期：2018-04-05

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