Isolation of Imaqobactin, an Amphiphilic Siderophore from the Arctic Marine Bacterium Variovorax Species RKJM285,Journal of Natural Products

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Isolation of Imaqobactin, an Amphiphilic Siderophore from the Arctic Marine Bacterium Variovorax Species RKJM285
Journal of Natural Products ( IF 3.3 ) Pub Date : 2018-04-04 00:00:00 , DOI: 10.1021/acs.jnatprod.7b00943
Andrew W. Robertson _{1,

2} , Nicholas G. McCarville ₂ , Logan W. MacIntyre ₃ , Hebelin Correa ₂ , Brad Haltli _{1,

2,

3} , Douglas H. Marchbank _{1,

2} , Russell G. Kerr _{1,

2,

3}

Affiliation

The amphiphilic siderophore imaqobactin was isolated from the Arctic bacterium Variovorax sp. RKJM285, a strain isolated from marine sediment collected from an inlet near Clyde River, Nunavut, Canada. The 2D structure of imaqobactin was determined by a combination of LC-HRMS, MS/MS, and NMR spectroscopic methods. The absolute configuration of the depsipeptide core was determined by Marfey’s analysis, and the relative configuration of the 4,7-diamino-3-hydroxy-2-methylheptanoic acid moiety was determined by NOESY and selective NOE experiments. The photoreductive properties of imaqobactin were tested and are discussed. Initial tests for antimicrobial and cytotoxic activity of imaqobactin were also performed, identifying moderate antimicrobial activity.

中文翻译：

从北极海洋细菌Variovorax物种RKJM285分离两亲铁载体伊马卡宾菌素

从北极细菌Variovorax sp。分离出两亲性铁载体imaqobactin 。RKJM285，一种从加拿大努纳武特州克莱德河附近的一个进口处收集的海洋沉积物中分离出的菌株。imaqobactin的2D结构是通过LC-HRMS，MS / MS和NMR光谱法的组合确定的。十肽核心的绝对构型是通过Marfey分析确定的，而4,7-二氨基-3-羟基-2-甲基庚酸部分的相对构型是通过NOESY和选择性NOE实验确定的。测试并讨论了伊马卡贝汀的光还原特性。还进行了伊马曲菌素的抗微生物和细胞毒性活性的初步测试，确定了中等的抗微生物活性。

更新日期：2018-04-04

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