This work describes the synthesis and characterization of pH-responsive hydrogels based on alginate for protein delivery. Synthesis approach shown here has included the modification of sodium alginate to convert it into a covalently crosslinkable polysaccharide and the subsequent radical polymerization reaction with sodium acrylate and N-vinylpyrrolidone for hydrogelation. To evaluate the applicability of the obtained hydrogels as an oral protein delivery system, we studied the cytotoxicity, the drug release profile (using BSA as a protein model), and the swelling performance in the basic and acidic environments. The hydrogels showed a pH-dependent swelling profile with higher value at pH 7.4. The protein release mechanism was demonstrated to be dependent on pH and composition. The proposed materials were shown to be compatible with living cells, indicating great pharmacological potential. These results show that the hydrogels are ideally suited for use as an oral drug delivery device.

这项工作描述了基于藻酸盐用于蛋白质递送的pH响应水凝胶的合成和表征。此处显示的合成方法包括修饰海藻酸钠以将其转化为可共价交联的多糖，以及随后与丙烯酸钠和N的自由基聚合反应-乙烯基吡咯烷酮用于水凝胶化。为了评估获得的水凝胶作为口服蛋白质递送系统的适用性，我们研究了在碱性和酸性环境下的细胞毒性，药物释放曲线（使用BSA作为蛋白质模型）以及溶胀性能。水凝胶显示出pH依赖性的溶胀曲线，在pH 7.4时具有较高的值。已证明蛋白质释放机理取决于pH和组成。所提出的材料显示与活细胞相容，表明具有巨大的药理潜力。这些结果表明水凝胶非常适合用作口服药物递送装置。