Chemical investigation on the deep-sea-derived fungus Penicillium granulatum MCCC 3A00475 led to the isolation of a previously undescribed (roquefortine J, 1) and four known (2-5) roquefortine alkaloids, along with six ergosterol analogues (6-11). The planar structure of 1 was established mainly on the basis of extensive analysis of its 1D, 2D NMR, and HRESIMS spectra. The absolute configuration of 1 was determined by comparison of the calculated and experimental electronic circular dichroism spectra. Compounds 5, 6, and 7 exhibited potent anti-proliferative effects against HepG2 tumor cells with IC₅₀ values of 7.0, 8.6, and 8.2 μM, respectively.

中文翻译：

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Roquefortine J，一种新颖的roquefortine生物碱，来自深海真菌颗粒青霉MCCC 3A00475。

对深海真菌颗粒青霉MCCC 3A00475的化学研究导致分离出先前未描述的（roquefortine J，1）和四个已知的（2-5）roquefortine生物碱，以及六个麦角固醇类似物（6-11）。1的平面结构主要是基于对其1D，2D NMR和HRESIMS光谱的广泛分析而建立的。通过比较计算的和实验的电子圆二色性光谱，确定1的绝对构型。化合物5、6和7对HepG2肿瘤细胞表现出有效的抗增殖作用，IC ₅₀值分别为7.0、8.6和8.2μM。