当前位置：
X-MOL 学术
›
ACS Sustain. Chem. Eng.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Converting Spent Coffee Grounds into Bioactive Extracts with Potential Skin Antiaging and Lightening Effects
ACS Sustainable Chemistry & Engineering ( IF 7.1 ) Pub Date : 2018-04-03 00:00:00 , DOI: 10.1021/acssuschemeng.8b00108 Helena M. Ribeiro 1 , Margherita Allegro 1 , Joana Marto 1 , Bruno Pedras 2 , Nuno G. Oliveira 1 , Alexandre Paiva 2 , Susana Barreiros 2 , Lídia M. Gonçalves 1 , Pedro Simões 2
ACS Sustainable Chemistry & Engineering ( IF 7.1 ) Pub Date : 2018-04-03 00:00:00 , DOI: 10.1021/acssuschemeng.8b00108 Helena M. Ribeiro 1 , Margherita Allegro 1 , Joana Marto 1 , Bruno Pedras 2 , Nuno G. Oliveira 1 , Alexandre Paiva 2 , Susana Barreiros 2 , Lídia M. Gonçalves 1 , Pedro Simões 2
Affiliation
|
The aim of this study was to valorize spent coffee grounds (SCG) into bioactive extracts for improving skin health. These extracts were obtained by subcritical water extraction (SWE) at 100 bar and temperatures up to 220 °C, in a semicontinuous mode. They were analyzed for phenolic acids, carbohydrates, antioxidant activity (AA) measured by the DPPH assay, ROS-scavenging activity in keratinocyte cells, and elastase and tyrosinase inhibitory activity. SCG extracts collected up to 140 °C had higher phenolic acids content (19.9 mgGA/gdry SCG), higher AA (EC50 of 20.6 μg mL–1), and lower carbohydrate content (38 mgsugars/gdry SCG) than the fraction collected from 140 to 220 °C (5.7 mgGA/gdry SCG, EC50 of 132.2 μg mL–1, and 286 mgsugars/gdry SCG). The extracts were proven to have antiaging and skin lightening effects by inhibiting elastase (99% and 97.9%) and tyrosinase (78.6% and 92.1%) activity. The extracts were incorporated in shear thinning and acidic hydrogels for topical application. Release (70%) and permeation studies (65.3 ± 13.1 μg/cm2) as well as cytotoxicity assays were performed to evaluate the release and permeation of bioactive compounds and the safety of hydrogels.
中文翻译：
将用过的咖啡渣转化为具有生物活性的提取物，具有潜在的皮肤抗衰老和美白功效
这项研究的目的是将用过的咖啡渣（SCG）制成具有生物活性的提取物，以改善皮肤健康。这些提取物是通过半临界模式在100 bar和最高220°C的温度下通过亚临界水提取（SWE）获得的。分析了它们的酚酸，碳水化合物，通过DPPH测定测得的抗氧化活性（AA），角质形成细胞中的ROS清除活性以及弹性蛋白酶和酪氨酸酶抑制活性。收集到的最高温度为140°C的SCG提取物的酚酸含量（19.9 mg GA / g干SCG），较高的AA（EC 50为20.6μgmL –1）和较低的碳水化合物含量（38 mg糖/ g SCG）从140到220°C（5.7 mgGA / g干SCG，EC 50为132.2μgmL –1和286 mg糖/ g干SCG）。通过抑制弹性蛋白酶（99％和97.9％）和酪氨酸酶（78.6％和92.1％）活性，证明提取物具有抗衰老和亮肤作用。将提取物掺入剪切稀化和酸性水凝胶中以局部使用。进行了释放（70％）和渗透研究（65.3±13.1μg/ cm 2）以及细胞毒性测定，以评估生物活性化合物的释放和渗透以及水凝胶的安全性。
更新日期：2018-04-03
中文翻译：
将用过的咖啡渣转化为具有生物活性的提取物，具有潜在的皮肤抗衰老和美白功效
这项研究的目的是将用过的咖啡渣（SCG）制成具有生物活性的提取物，以改善皮肤健康。这些提取物是通过半临界模式在100 bar和最高220°C的温度下通过亚临界水提取（SWE）获得的。分析了它们的酚酸，碳水化合物，通过DPPH测定测得的抗氧化活性（AA），角质形成细胞中的ROS清除活性以及弹性蛋白酶和酪氨酸酶抑制活性。收集到的最高温度为140°C的SCG提取物的酚酸含量（19.9 mg GA / g干SCG），较高的AA（EC 50为20.6μgmL –1）和较低的碳水化合物含量（38 mg糖/ g SCG）从140到220°C（5.7 mgGA / g干SCG，EC 50为132.2μgmL –1和286 mg糖/ g干SCG）。通过抑制弹性蛋白酶（99％和97.9％）和酪氨酸酶（78.6％和92.1％）活性，证明提取物具有抗衰老和亮肤作用。将提取物掺入剪切稀化和酸性水凝胶中以局部使用。进行了释放（70％）和渗透研究（65.3±13.1μg/ cm 2）以及细胞毒性测定，以评估生物活性化合物的释放和渗透以及水凝胶的安全性。
京公网安备 11010802027423号