AbstractA nanoporous coordination polymer (NPCP) was prepared from palladium(II) chloride and 4-amino-3-hydrazino-5-mercapto-1,2,4-triazole and is shown to act as a peroxidase mimetic. It can catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) by H2O2 which is formed on enzymatic oxidation of glucose by glucose oxidase. Based on these findings, a sensitive glucose test was worked out at 652 nm where the intensity if the greenish-blue product is related to the actual concentration of glucose. Figures of merit include (a) rather low Km value (30 μM) which evidences the strong binding affinity of the NPCP toward glucose, (b) a high v(max) (8.5 M·s−1), (c) a 47 nM detection limit, (d) a lifetime of a month, (e) a wide working pH range (2–10), and (f) a 25–80 °C temperature range. The assay was applied to non-invasive determination of glucose assay in tear, saliva where the detection limits are found to be 61 and 91 nM, respectively. Graphical abstractDSchematic of the mechanism of the peroxidase like catalytic activity of AHMT-Pd NPCP that was applied in a selective colorimetric method for glucose detection based on TMB oxidation in the presence of enzymatically generated H2O2.

中文翻译：

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一种纳米多孔钯 (II) 桥连配位聚合物作为过氧化物酶模拟物，用于视觉检测泪液和唾液中的葡萄糖

摘要 以氯化钯 (II) 和 4-amino-3-hydrazino-5-mercapto-1,2,4-triazole 制备纳米多孔配位聚合物 (NPCP)，并显示其可作为过氧化物酶模拟物。它可以通过葡萄糖氧化酶对葡萄糖进行酶促氧化形成的 H2O2 催化 3,3',5,5'-四甲基联苯胺 (TMB) 的氧化。基于这些发现，在 652 nm 处进行了灵敏的葡萄糖测试，其中绿蓝色产物的强度与葡萄糖的实际浓度相关。品质因数包括 (a) 相当低的 Km 值 (30 μM)，这证明了 NPCP 对葡萄糖的强结合亲和力，(b) 高 v(max) (8.5 M·s-1)，(c) a 47 nM 检测限，(d) 一个月的寿命，(e) 宽的工作 pH 范围 (2-10)，以及 (f) 25-80 °C 的温度范围。该测定应用于眼泪、唾液中葡萄糖测定的非侵入性测定，其中发现检测限分别为 61 和 91 nM。图形摘要D过氧化物酶的机制示意图，如 AHMT-Pd NPCP 的催化活性，其在酶促生成的 H2O2 存在下应用于基于 TMB 氧化的葡萄糖检测选择性比色方法。