Abstract The study aims to investigate the antagonistic ability of Lactobacillus plantarum 0612 against foodborne enteropathogens adhesion to the human intestinal Caco-2 cells by competition in a simulated colon model. L. plantarum 0612 has been subjected to the gastrointestinal transits simulation (GITS) prior to assess its competitive inhibition on the adhesion of selected pathogenic strains using intestinal epithelial cells with different colonic pH conditions. Results showed that the adhesion of E. coli and L. monocytogenes on Caco-2 cells was significantly inhibited by L. plantarum 0612 with 4.35 and 4.14 log reductions, respectively. Exclusion and competition mechanisms seemed to be more effective in preventing the colonization of pathogens, indicating L. plantarum 0612 is able to compete for receptors of the epithelial cell surfaces. The GITS tolerance L. plantarum 0612 exhibited stronger competitive inhibition (p

中文翻译：

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植物乳杆菌 0612 对不同结肠环境中选定食源性肠道病原体粘附的拮抗作用

摘要 本研究旨在通过模拟结肠模型竞争研究植物乳杆菌0612对抗食源性肠道病原体粘附在人肠道Caco-2细胞上的拮抗能力。在使用具有不同结肠 pH 条件的肠上皮细胞评估其对选定致病菌株粘附的竞争性抑制之前，植物乳杆菌 0612 已接受胃肠传输模拟 (GITS)。结果表明，大肠杆菌和单核细胞增生李斯特菌对 Caco-2 细胞的粘附受到植物乳杆菌 0612 的显着抑制，分别降低了 4.35 和 4.14 log。排斥和竞争机制似乎更有效地防止病原体定植，表明植物乳杆菌 0612 能够竞争上皮细胞表面的受体。