The present study aimed to gain better insights into profitable biomethanation through sodium thiosulphate induced immobilized protease secreting bacterial disintegration (STS-IPBD) of sludge. STS disperse the flocs at 0.08 g/g SS of dosage and assists the subsequent bacterial disintegration. Immobilization of bacteria increases the hydrolytic activity of cells towards effective liquefaction of sludge. A higher liquefaction of 22% was accomplished for STS-IPBD when compared to immobilized protease secreting bacterial disintegration (IPBD alone). The kinetic parameters of Line Weaver Burk plot analysis revealed a maximal specific growth rate (µmax) of 0.320 h for immobilized cells when compared to suspended free cells showing the benefit of immobilization. Floc dispersion and immobilization of bacteria imparts a major role in biomethanation as the methane generation (0.32 gCOD/g COD) was higher in STS-IPBD sample. The cost analysis showed that STS – IPBD was a feasible process with net profit of 2.6 USD/Ton of sludge.

中文翻译：

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硫代硫酸钠诱导污泥固定化细菌分解：污泥管理和生物甲烷化的节能且经济高效的平台


本研究旨在通过硫代硫酸钠诱导污泥固定化蛋白酶分泌细菌崩解（STS-IPBD）更好地了解有利可图的生物甲烷化。 STS 以 0.08 g/g SS 剂量分散絮凝物，并协助随后的细菌分解。细菌的固定化增加了细胞的水解活性，从而实现污泥的有效液化。与分泌细菌崩解的固定化蛋白酶（单独的 IPBD）相比，STS-IPBD 的液化率更高，达到 22%。 Line Weaver Burk 图分析的动力学参数显示，与悬浮的游离细胞相比，固定化细胞的最大比生长速率 (μmax) 为 0.320 小时，显示出固定化的优势。细菌的絮体分散和固定在生物甲烷化中发挥着重要作用，因为 STS-IPBD 样品中的甲烷生成量 (0.32 gCOD/g COD) 较高。成本分析表明，STS-IPBD工艺可行，净利润为2.6美元/吨污泥。