Conjugated Polymer with Aggregation-Directed Intramolecular Förster Resonance Energy Transfer Enabling Efficient Discrimination and Killing of Microbial Pathogens,Chemistry of Materials

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Conjugated Polymer with Aggregation-Directed Intramolecular Förster Resonance Energy Transfer Enabling Efficient Discrimination and Killing of Microbial Pathogens
Chemistry of Materials ( IF 7.2 ) Pub Date : 2018-03-29 00:00:00 , DOI: 10.1021/acs.chemmater.8b00164
Shuxian Zhu ₁ , Xiaoyu Wang ₁ , Yu Yang ₁ , Haotian Bai ₂ , Qianling Cui ₁ , Han Sun ₂ , Lidong Li _{1,

3} , Shu Wang ₂

Affiliation

Rapid and effective differentiation and killing of microbial pathogens are major challenges in the diagnosis and treatment of infectious diseases. Here, we report a novel system based on the conjugated polymer poly[(9,9-bis{6′-[N-(triethylene glycol methyl ether)-di(1H-imidazolium)methane]hexyl}-2,7-fluorene)-co-4,7-di-2-thienyl-2,1,3-benzothiadiazole] tetrabromide (PFDBT-BIMEG), which enables efficient microbial pathogen discrimination and killing. The functional side chains of PFDBT-BIMEG enabled both electrostatic and salt bridge interactions with microorganisms. Microorganism binding events caused a change in the aggregation structure of PFDBT-BIMEG, which could be recognized by a change of its fluorescence signal by intramolecular Förster resonance energy transfer (FRET). This sensing strategy allowed rapid and sensitive distinction of microbial pathogens within 15 min. We performed linear discrimination analysis that featured this advance to confirm that the polymer PFDBT-BIMEG could accurately classify microbial pathogens. Owing to the different adhesion mechanism of PFDBT-BIMEG to the surface of the microorganisms, we applied different sterilization strategies for each kind of microbial pathogen. The microbial pathogens could be efficiently killed by reactive oxygen species produced from PFDBT-BIMEG under irradiation, avoiding the use of any other antibacterial agents. This methodology, which combines pathogen discrimination and killing, represents a promising alternative to current diagnostic platforms.

中文翻译：

共轭聚合物与聚集定向分子内弗斯特共振能量转移，能够有效区分和杀死微生物病原体

快速有效地分化和杀死微生物病原体是传染病诊断和治疗的主要挑战。在这里，我们报告基于共轭聚合物聚[（9,9-双{6'-[ N-（三甘醇甲基醚）-二（1 H-咪唑鎓）甲烷]己基} -2,7-芴）-co-4,7-二-2-噻吩基-2,1,3-苯并噻二唑]四溴化物（PFDBT-BIMEG），可有效区分和杀死微生物。PFDBT-BIMEG的功能性侧链可实现与微生物的静电和盐桥相互作用。微生物结合事件导致PFDBT-BIMEG的聚集结构发生变化，这可以通过分子内Förster共振能量转移（FRET）的荧光信号变化来识别。这种传感策略允许在15分钟内快速，灵敏地区分微生物病原体。我们进行了以这一进展为特征的线性判别分析，以确认聚合物PFDBT-BIMEG可以准确地对微生物病原体进行分类。由于PFDBT-BIMEG对微生物表面的粘附机制不同，我们对每种微生物病原体采用了不同的灭菌策略。PFDBT-BIMEG在辐射下产生的活性氧可有效杀死微生物病原体，而无需使用任何其他抗菌剂。这种结合了病原体识别和杀死功能的方法论是当前诊断平台的有前途的替代方法。

更新日期：2018-03-29

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