A nitroreductase and acidity detecting dual functional ratiometric fluorescent probe for selectively imaging tumor cells†,Organic & Biomolecular Chemistry

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A nitroreductase and acidity detecting dual functional ratiometric fluorescent probe for selectively imaging tumor cells†
Organic & Biomolecular Chemistry ( IF 2.9 ) Pub Date : 2018-03-29 00:00:00 , DOI: 10.1039/c8ob00670a
Zhaoshuai He _{1,

2,

3,

4,

5} , Yajie Chou _{1,

2,

3,

4,

5} , Hanxin Zhou _{1,

2,

3,

4,

5} , Han Zhang _{1,

2,

3,

4,

5} , Tanyu Cheng _{1,

2,

3,

4,

5} , Guohua Liu _{1,

2,

3,

4,

5}

Affiliation

Nitroreductase and acidity are common features of a tumor, which is associated with serious disease with a high mortality rate. However, solo detection of nitroreductase or acidic environments may cause false-positive results. Thus, it is very meaningful to develop dual functional probes for detecting nitroreductase and acidity, as these could be used for accurate tumor imaging. Here we report on the first dual functional ratiometric fluorescent probe, NAFP, for acidity and nitroreductase detection. This probe enables the measurement of the acidic microenvironment and the concentration of nitroreductase at the same time and the same site. The probe can obviously distinguish acidity, nitroreductase, and nitroreductase in an acidic environment. Furthermore, confocal fluorescence imaging of A549 cells indicates that NAFP can detect acidity and expressed nitroreductase in living cells.

中文翻译：

氮还原酶和酸度检测双功能比率荧光探针，用于对肿瘤细胞进行选择性成像†

硝基还原酶和酸性是肿瘤的共同特征，与严重的疾病和高死亡率相关。但是，单独检测硝基还原酶或酸性环境可能会导致假阳性结果。因此，开发用于检测硝基还原酶和酸性的双功能探针非常有意义，因为它们可用于精确的肿瘤成像。在这里，我们报告第一个双功能比例荧光探针NAFP，用于酸度和硝基还原酶检测。该探针可在同一时间和同一位置测量酸性微环境和硝基还原酶的浓度。该探针可以明显区分酸性环境中的酸度，硝基还原酶和硝基还原酶。此外，A549细胞的共聚焦荧光成像表明，NAFP可以检测活细胞中的酸度并表达硝基还原酶。

更新日期：2018-03-29

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