Micropassage-embedding composite hydrogel fibers enable quantitative evaluation of cancer cell invasion under 3D coculture conditions†,Lab on a Chip

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Micropassage-embedding composite hydrogel fibers enable quantitative evaluation of cancer cell invasion under 3D coculture conditions†
Lab on a Chip ( IF 6.1 ) Pub Date : 2018-03-29 00:00:00 , DOI: 10.1039/c7lc01280b
Manami Sugimoto _{1,

2,

3,

4,

5} , Yoichi Kitagawa _{1,

2,

3,

4,

5} , Masumi Yamada _{1,

2,

3,

4,

5} , Yuya Yajima _{1,

2,

3,

4,

5} , Rie Utoh _{1,

2,

3,

4,

5} , Minoru Seki _{1,

2,

3,

4,

5}

Affiliation

Cell migration and invasion are of significant importance in physiological phenomena, including wound healing and cancer metastasis. Here we propose a new system for quantitatively evaluating cancer cell invasion in a three-dimensional (3D), in vivo tissue-like environment. This system uses composite hydrogel microfibers whose cross section has a relatively soft micropassage region and that were prepared using a multilayered microfluidic device; cancer cells are encapsulated in the core and fibroblasts are seeded in the shell regions surrounding the core. Cancer cell proliferation is guided through the micropassage because of the physical restriction imposed by the surrounding solid shell regions. Quantitative analysis of cancer cell invasion is possible simply by counting the cancer cell colonies that form outside the fiber. This platform enables the evaluation of anticancer drug efficacy (cisplatin, paclitaxel, and 5-fluorouracil) based on the degree of invasion and the gene expression of cancer cells (A549 cells) with or without the presence of fibroblasts (NIH-3T3 cells). The presented hydrogel fiber-based migration assays could be useful for studying cell behaviors under 3D coculture conditions and for drug screening and evaluation.

中文翻译：

微通道嵌入复合水凝胶纤维可在3D共培养条件下定量评估癌细胞的侵袭性†

细胞迁移和侵袭在包括伤口愈合和癌症转移在内的生理现象中具有重要意义。在这里，我们提出了一种新的系统，用于在体内三维（3D）中定量评估癌细胞的侵袭类组织环境。该系统使用复合水凝胶微纤维，其横截面具有相对较软的微通道区域，并且是使用多层微流体装置制备的。癌细胞被包裹在核心中，成纤维细胞被植入核心周围的壳区域。由于周围的固体外壳区域施加了物理限制，因此癌细胞的增殖通过微通道引导。只需对纤维外部形成的癌细胞集落进行计数，就可以对癌细胞的侵袭进行定量分析。该平台能够基于有或没有成纤维细胞（NIH-3T3细胞）的癌细胞（A549细胞）的侵袭程度和基因表达，评估抗癌药功效（顺铂，紫杉醇和5-氟尿嘧啶）。

更新日期：2018-03-29

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