The binding of titanocene to DNA and RNA was examined by means of electrospray mass spectrometry. Titanocene served as a model for its therapeutically active derivatives. The binding preferences were probed by competition experiments with oligonucleotides of varying nucleobase compositions and sequences. Results from competition experiments revealed a generally increased preference for the binding to phosphate groups adjacent to thymidines, which is affected by the nucleobase sequence of T-rich oligonucleotides. More detailed information about the binding sites was obtained from tandem mass spectrometric experiments. The binding of the transition metal coordination center significantly altered the fragment ion patterns of the oligonucleotides. RNA was found to be less prone to adduct formation, due to intramolecular interactions. The findings from experiments on DNA and RNA were complemented by the examination of backbone- and ribose-modified oligonucleotides.

通过电喷雾质谱法检测钛茂与DNA和RNA的结合。钛新世为其治疗活性衍生物的模型。通过竞争实验用变化的核碱基组成和序列的寡核苷酸探测结合偏好。竞争实验的结果表明，与富含胸腺嘧啶核苷的磷酸基团结合的偏好通常会增加，这受富含T的寡核苷酸的碱基序列影响。关于结合位点的更详细的信息是从串联质谱实验中获得的。过渡金属配位中心的结合显着改变了寡核苷酸的碎片离子模式。由于分子内相互作用，发现RNA不太容易形成加合物。