In this study, we developed a high-throughput antifungal activity screening method using a cheese-mimicking matrix distributed in 24-well plates. This method allowed rapid screening of a large variety of antifungal agent candidates: bacterial fermented ingredients, bacterial isolates, and preservatives. Using the proposed method, we characterized the antifungal activity of 44 lactic acid bacteria (LAB) fermented milk-based ingredients and 23 LAB isolates used as protective cultures against 4 fungal targets (Mucor racemosus, Penicillium commune, Galactomyces geotrichum, and Yarrowia lipolytica). We also used this method to determine the minimum inhibitory concentration of a preservative, natamycin, against 9 fungal targets. The results underlined the strain-dependency of LAB antifungal activity, the strong effect of fermentation substrate on this activity, and the effect of the screening medium on natamycin minimum inhibitory concentration. Our method could achieved a screening rate of 1,600 assays per week and can be implemented to evaluate antifungal activity of microorganisms, fermentation products, or purified compounds compatible with dairy technology.

在这项研究中，我们开发了一种高通量抗真菌活性筛选方法，使用的奶酪模拟基质分布在24孔板中。这种方法可以快速筛选多种抗真菌剂候选物：细菌发酵成分，细菌分离物和防腐剂。使用所提出的方法，我们的特点的44个乳酸菌（LAB）发酵的乳基成分和23 LAB中的抗真菌活性菌株用作抗真菌4个靶保护培养物（总状毛霉，青霉，Galactomyces白地霉，和脂耶氏酵母）。我们还使用这种方法来确定防腐剂那他霉素对9种真菌靶标的最低抑菌浓度。该结果强调了LAB抗真菌活性的菌株依赖性，发酵底物对该活性的强作用以及筛选培养基对那他霉素最小抑制浓度的影响。我们的方法每周可实现1,600次测定的筛选率，并可用于评估微生物，发酵产品或与乳制品技术兼容的纯化化合物的抗真菌活性。