This paper presents a whole-cell biosensor that operates in conjunction with a smartphone-based fluorescence diagnostic system on a paper device to monitor the concentration of gold ions in human urine. The heavy metal-tolerant bacteria Cupriavidus metallidurans was genetically engineered for use as a chassis in a red fluorescent protein (RFP)-based microbial sensor. The biosensor is highly sensitive to gold ions, with a detection limit of 110 nM. The proposed smartphone-based analysis system provides a user-friendly approach to design tools of personal health monitoring for reporting the presence of gold ions in human urine.

中文翻译：

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在纸质装置上使用基因工程微生物测定人体尿液中的金离子


本文提出了一种全细胞生物传感器，该传感器与纸质设备上基于智能手机的荧光诊断系统结合使用，以监测人体尿液中金离子的浓度。耐重金属细菌Cupriavidus metallidurans经过基因工程改造，可用作基于红色荧光蛋白 (RFP) 的微生物传感器的底盘。该生物传感器对金离子高度敏感，检测限为 110 nM。所提出的基于智能手机的分析系统提供了一种用户友好的方法来设计个人健康监测工具，以报告人体尿液中金离子的存在。