Abstract A new, highly selective, sensitive and stable enzymatic glucose sensor was fabricated on glassy carbon electrode (GCE) using zinc oxide (ZnO) nanoparticles embedded nitrogen-doped carbon sheets (ZnO@NDCS), glucose oxidase (GOx) (assigned as GCE/ZnO@NDCS/GOx). First, ZnO@NDCS were synthesized by a simple hydrothermal method. Zn powder, aqueous ammonia, and peach extract were served as the precursor for ZnO NPs, nitrogen and carbon, respectively. The fabricated GCE/ZnO@NDCS/GOx biosensor exhibited a high and reproducible sensitivity of 231.7 μA mM−1 cm−2. Also, showed a wide linear range from 0.2 to 12 mM with a correlation coefficient R2 = 0.998 and lowest detection limit (based on S/N ratio = 3) of 6.3 μM. The GCE/ZnO@NDCS/GOx biosensor is acceptably stable, selective, and it was successfully applied to the quantitative monitoring of glucose in human blood serum. The synthesized ZnO@NDCS nanocomposite may be found useful in other applications in the fields of solar cells and optoelectronic devices. These encouraging results suggest a simple and effective method obtain electrode material for the enzymatic glucose sensor.

中文翻译：

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基于绿色合成氧化锌纳米颗粒嵌入氮掺杂碳片的高性能葡萄糖生物传感器

摘要 使用氧化锌 (ZnO) 纳米粒子嵌入氮掺杂碳片 (ZnO@NDCS)、葡萄糖氧化酶 (GOx) (指定为 GCE) 在玻碳电极 (GCE) 上制造了一种新型、高选择性、灵敏且稳定的酶促葡萄糖传感器。 /ZnO@NDCS/GOx)。首先，通过简单的水热法合成了 ZnO@NDCS。锌粉、氨水和桃提取物分别作为 ZnO NPs、氮和碳的前体。制造的 GCE/ZnO@NDCS/GOx 生物传感器表现出 231.7 μA mM-1 cm-2 的高且可重复的灵敏度。此外，还显示了从 0.2 到 12 mM 的宽线性范围，相关系数 R2 = 0.998，最低检测限（基于 S/N 比 = 3）为 6.3 μM。GCE/ZnO@NDCS/GOx 生物传感器具有可接受的稳定性、选择性、并成功应用于人血清中葡萄糖的定量监测。合成的 ZnO@NDCS 纳米复合材料可用于太阳能电池和光电器件领域的其他应用。这些令人鼓舞的结果表明了一种简单有效的方法来获得用于酶促葡萄糖传感器的电极材料。