Two new cytochalasans flavichalasine N (1), flavichalasine O (2), together with six known cytochalasans (3–8), were isolated from Aspergillus flavipes PJ03-11 through the application of OSMAC (one strain many compounds) strategy. Flavichalasine O (2) represented the first example of cytochalasans possessing a nitrogen-oxygen heterocycle at the macrocyclic ring part. Their structures were established on the base of extensive spectroscopic analysis. Compounds 1–4 exhibited significant cytotoxic activities against three human cancer cell lines (THP1, HL-60 and PC3) with IC₅₀ values ranging from 3.00 to 15.10 μM.

两个新的cytochalasans flavichalasine N（1），flavichalasine O（2具有六个已知cytochalasans（），一起3 - 8），从分离黄柄曲霉通过OSMAC（一种菌株许多化合物）策略的应用PJ03-11。Flavichalasine O（2）代表在大环部分具有氮-氧杂环的细胞松弛素的第一个例子。它们的结构是在广泛的光谱分析的基础上建立的。化合物1 - 4个针对三种人类肿瘤细胞系（THP1，HL-60和PC3）与IC表现出显著细胞毒活性₅₀ 值范围为3.00至15.10μM。