AbstractThe authors introduce a new kind of surface artificial biomimetic receptor, referred to as aptameric imprinted polymer (AIP), for separation of biological macromolecules. Highly dispersed magnetic nanoparticles (MNPs) were coated with silica and then functionalized with methacrylate groups via silane chemistry. The aptamer was covalently immobilized on the surface of nanoparticles via a “thiol-ene” click reaction. Once the target analyte (bovine serum albumin; BSA) has bound to the aptamer, a polymer is created by 2-dimensional copolymerization of short-length poly(ethylene glycol) and (3-aminopropyl)triethoxysilane. Following removal of BSA from the polymer, the AIP-MNPs presented here can selectively capture BSA with a specific absorbance (κ) as high as 65. When using this AIP, the recovery of BSA from spiked real biological samples is >97%, and the adsorption capacity is as high as 146 mg g−1. In our perception, this method has a wide scope in that it may be applied to the specific extraction of numerous other biomolecules. Graphical abstractSchematic presentation of the AIP (aptamer-imprinted polymer) introduced here. The surface of silica coated magnetic nanoparticles is modified with a polymer that is covalently modified with an aptamer against bovine serum albumin (BSA).

中文翻译：

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用于高通量蛋白质识别的磁性纳米颗粒表面印记的纳米尺寸适体腔

摘要 作者介绍了一种新型的表面人工仿生受体，称为适体印迹聚合物（AIP），用于生物大分子的分离。高度分散的磁性纳米粒子 (MNP) 用二氧化硅包覆，然后通过硅烷化学用甲基丙烯酸酯基团进行功能​​化。适配体通过“硫醇-烯”点击反应共价固定在纳米颗粒的表面。一旦目标分析物（牛血清白蛋白；BSA）与适体结合，就会通过短链聚（乙二醇）和（3-氨基丙基）三乙氧基硅烷的二维共聚产生聚合物。从聚合物中去除 BSA 后，此处介绍的 AIP-MNP 可以选择性地捕获比吸光度 (κ) 高达 65 的 BSA。使用此 AIP 时，从加标的真实生物样品中 BSA 的回收率 >97%，吸附容量高达 146 mg g-1。在我们看来，这种方法的范围很广，因为它可以应用于许多其他生物分子的特定提取。此处介绍的 AIP（适体印迹聚合物）的图形摘要示意图。二氧化硅涂覆的磁性纳米粒子的表面用聚合物改性，该聚合物用抗牛血清白蛋白 (BSA) 的适体共价改性。