Pestalustaines A (1) and B (2), one unique sesquiterpene possessing an unusual 5/6/7-fused tricyclic ring system and one unprecedented coumarin derivative bearing 6/6/5/5-fused tetracyclic ring system, were isolated from the plant-derived Pestalotiopsis adusta. Their structures with absolute configurations were established by extensive NMR analysis, X-ray crystallography, and CD spectra associated with TD-DFT calculation. Hypothetical biosynthetic pathways for compounds 1 and 2 are proposed. Compounds 1 and 2 showed weak to moderate cytotoxic activities against three human tumor cell lines HeLa, HCT116, and A549, whose IC₅₀ values were ranged from 21.01 to 55.43 μM.

从分离出了Pestalustaines A（1）和B（2），一种具有不同寻常的5/6/7稠合三环体系的独特倍萜烯和一种具有6/6/5/5稠合四环体系的香豆素衍生物。植物来源的拟青霉。通过广泛的NMR分析，X射线晶体学和与TD-DFT计算相关的CD光谱，确定了它们具有绝对构型的结构。提出了化合物1和2的假设生物合成途径。化合物1和2对三种人类肿瘤细胞HeLa，HCT116和A549的IC ₅₀表现出弱至中等的细胞毒活性 值范围为21.01至55.43μM。