Bioaccessible selenium sourced from Se-rich mustard cake facilitates protection from TBHP induced cytotoxicity in melanoma cells,Food & Function

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Bioaccessible selenium sourced from Se-rich mustard cake facilitates protection from TBHP induced cytotoxicity in melanoma cells
Food & Function ( IF 5.1 ) Pub Date : 2018-03-26 00:00:00 , DOI: 10.1039/c7fo01644a
Sumit K. Jaiswal _{1,

2,

3,

4} , Ranjana Prakash _{2,

3,

4,

5} , K. Sandeep Prabhu _{6,

7,

8,

9,

10} , N. Tejo Prakash _{1,

2,

3,

4}

Affiliation

Selenium (Se) is an essential dietary supplement that resolves inflammatory responses and offers antioxidant cytoprotection. In this study, we present the data on the cytoprotective effect of Se-rich mustard protein isolated from mustard cultivated in seleniferous soils in Punjab, India. The concentrations of total Se in mustard seed, oil-free mustard cake, and mustard protein were 110.0 ± 3.04, 143.0 ± 5.18, and 582.3 ± 6.23 μg g⁻¹, respectively. The cytoprotective effect of Se-rich mustard protein was studied on tert-butyl hydroperoxide (TBHP)-induced cytotoxicity in a mouse melanoma cell line (B16-F10). When compared with TBHP treated cells (where no viable cells were found), Se-rich protein made bioaccessible through simulated gastrointestinal digestion protected melanoma cells from cytotoxicity with decreased levels of oxidative stress resulting in 73% cell viability. Such an effect was associated with a significant increase in glutathione peroxidase activity as a function of bioaccessible Se and its response towards cytoprotection.

中文翻译：

来自富含硒的芥菜饼的可生物利用的硒促进了黑色素瘤细胞免受TBHP诱导的细胞毒性的保护

硒（Se）是一种基本的膳食补充剂，可解决炎症反应并提供抗氧化细胞保护作用。在这项研究中，我们提供了从印度旁遮普邦硒环境中种植的芥菜中分离出的富硒芥菜蛋白对细胞的保护作用数据。芥菜籽，无油芥菜饼和芥菜蛋白中总硒的浓度分别为110.0±3.04、143.0±5.18和582.3±6.23μgg ^-1。富硒芥菜蛋白质的细胞保护作用进行了研究对叔丁基过氧化氢（TBHP）诱导的小鼠黑素瘤细胞系（B16-F10）的细胞毒性。与TBHP处理的细胞（未发现活细胞）相比，富含Se的蛋白质可通过模拟获得生物可及性胃肠道消化保护黑素瘤细胞免受细胞毒性，同时氧化应激水平降低，导致细胞活力达到73％。这种作用与谷胱甘肽过氧化物酶活性的显着增加有关，后者是生物可及硒的功能及其对细胞保护的反应。

更新日期：2018-03-26

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