Abstract A rapid and effective pre-treatment method based on immune-magnetic separation (IMS) with enrichment culture was developed to rapidly detect trace levels of Salmonella . Immune-magnetic beads were prepared and their capture efficiency exceeded 90% with less than 10 6 CFU/mL of Salmonella . IMS was performed on 25-mL samples without pre-enrichment; instead, the trapped target pathogens were cultured in buffered peptone water for a short time. This pre-treatment was significantly shorter and simpler: the 5 h processing time included IMS for 1 h and enrichment for 4 h. Enzyme-linked immunosorbent assay, loop-mediated isothermal amplification test, and fluorescence quantitative real-time PCR in combination with the pre-treatment method allowed detection of 10 −1 CFU/mL of Salmonella (12.3 CFU in 25 mL of milk) in approximately 6.5–8 h. The pre-treatment method developed in this study was effective and can be applied to rapid screening of trace pathogens in liquid food samples.

中文翻译：

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基于免疫磁分离的有效前处理技术快速检测牛奶中痕量沙门氏菌

摘要 开发了一种基于免疫磁分离（IMS）和富集培养的快速有效的前处理方法，可快速检测痕量沙门氏菌。制备了免疫磁珠，其捕获效率超过90%，沙门氏菌小于10 6 CFU/mL。IMS 是在 25 mL 样品上进行的，没有预富集；相反，捕获的目标病原体在缓冲蛋白胨水中培养了很短的时间。这种预处理明显更短和更简单：5 小时的处理时间包括 1 小时的 IMS 和 4 小时的富集。酶联免疫吸附试验、环介导等温扩增试验和荧光定量实时 PCR 与预处理方法相结合，可检测约 10 -1 CFU/mL 的沙门氏菌（25 mL 牛奶中 12.3 CFU） 6.5-8 小时。