Ultrasonicated Lespedeza cuneata extract prevents TNF-α-induced early atherosclerosis in vitro and in vivo†,Food & Function

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Ultrasonicated Lespedeza cuneata extract prevents TNF-α-induced early atherosclerosis in vitro and in vivo†
Food & Function ( IF 5.1 ) Pub Date : 2018-03-26 00:00:00 , DOI: 10.1039/c7fo01666b
Su Jeong Ha _{1,

2,

3,

4,

5} , Jangho Lee _{1,

2,

3,

4,

6} , Kyung-Mo Song _{1,

2,

3,

4} , Young Ho Kim _{1,

2,

3,

4} , Nam Hyouck Lee _{1,

2,

3,

4} , Young-Eon Kim _{1,

2,

3,

4} , Sung Keun Jung _{1,

2,

3,

4,

7}

Affiliation

This study evaluated the use of ultrasonication to extract Lespedeza cuneata as a potential nutraceutical for preventing vascular inflammation. Ultrasonicated L. cuneata extract (ULCE) was prepared using 20% ethanol and 2 h of ultrasonication at room temperature, and its effects were investigated using relevant in vitro and in vivo models. ULCE suppressed tumor necrosis factor-alpha (TNF-α)-induced adhesion capacity, vascular cell adhesion protein 1 (VCAM-1) expression, and nuclear factor kappa-B (NF-κB) activity in human umbilical vein endothelial cells (HUVECs). ULCE also suppressed TNF-α-induced NF-κB signaling pathways and p65 translocation from the cytosol to the nucleus, as well as the mRNA expression of IL-1β, IL-6, and TNF-α in HUVECs. Oral administration of ULCE suppressed TNF-α-induced monocyte infiltration into the intima and VCAM-1 expression, as well as the IL-1β, IL-6, TNF-α, and monocyte chemoattractant protein-1 (MCP-1) mRNA expression in the main artery in mice. Among the compounds identified in the hydrolyzed ULCE, quercetin exhibited the strongest inhibitory effect against TNF-α-induced cell adhesion capacity. These results demonstrate that ULCE contains potent preventive factors against early atherosclerosis, which act by suppressing the NF-κB and VCAM-1 signaling axis.

中文翻译：

超声铁扫帚提取物防止TNF-α诱导的动脉粥样硬化的早期在体外和体内†

这项研究评估了超声提取提取Lespedeza cuneata作为预防血管炎症的潜在保健品的用途。用20％的乙醇和室温超声2 h制备了超声处理的库氏乳杆菌提取物（ULCE），并使用相关的体内和体外研究了其效果。楷模。ULCE抑制肿瘤坏死因子-α（TNF-α）诱导的人脐静脉内皮细胞（HUVEC）的粘附能力，血管细胞粘附蛋白1（VCAM-1）表达和核因子κB（NF-κB）活性。。ULCE还抑制了TNF-α诱导的NF-κB信号通路和p65从胞质到细胞核的移位，以及HUVEC中IL-1β，IL-6和TNF-α的mRNA表达。口服ULCE可抑制TNF-α诱导的单核细胞浸润到内膜和VCAM-1表达以及IL-1β，IL-6，TNF-α和单核细胞趋化蛋白1（MCP-1）mRNA表达在小鼠的主动脉中。在水解的ULCE中鉴定的化合物中，槲皮素对TNF-α诱导的细胞粘附能力表现出最强的抑制作用。

更新日期：2018-03-26

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