A Fluorescent Probe for Stimulated Emission Depletion Super-Resolution Imaging of Vicinal-Dithiol-Proteins on Mitochondrial Membrane,Bioconjugate Chemistry

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A Fluorescent Probe for Stimulated Emission Depletion Super-Resolution Imaging of Vicinal-Dithiol-Proteins on Mitochondrial Membrane
Bioconjugate Chemistry ( IF 4.0 ) Pub Date : 2018-03-23 00:00:00 , DOI: 10.1021/acs.bioconjchem.8b00128
Zhigang Yang _{1,

2} , Dong Hoon Kang ₃ , Hoyeon Lee ₄ , Jinwoo Shin ₂ , Wei Yan ₁ , Bhowmira Rathore ₁ , Hye-Ri Kim ₄ , Seo Jin Kim ₄ , Hardev Singh ₂ , Liwei Liu ₁ , Junle Qu ₁ , Chulhun Kang ₄ , Jong Seung Kim _{1,

2}

Affiliation

Realizing the significant roles of vicinal-dithiol proteins (VDPs) in maintaining the cellular redox homeostasis and their implication in many diseases, we synthesized a smart arsenate based fluorescent probe 1 which can preferentially target the mitochondrial membrane-bound vicinal dithiol proteins (VDPs), especially voltage-dependent anion channel (VDAC2). The probe targetability was demonstrated by in vitro studies such as colocalization, stimulated emission depletion (STED) super-resolution imaging, proteomic MS/MS analysis, and Western blot analysis. The probe represents a rare example of fluorescence labeling of mitochondrial membrane-bound VDPs and can provide a new way to construct VDPs-specific fluorescent probes to gain deeper understanding of their roles in mitochondrial-related disorders.

中文翻译：

用于线粒体膜上的邻二硫醇蛋白质刺激发射耗竭超高分辨率成像的荧光探针。

我们意识到邻域二硫醇蛋白（VDP）在维持细胞氧化还原稳态中的重要作用及其在许多疾病中的意义，我们合成了一种基于砷酸盐的智能荧光探针1，该探针可优先靶向线粒体膜结合的邻域二硫醇蛋白（VDP），尤其是依赖于电压的阴离子通道（VDAC2）。探针的可靶向性已通过体外研究证明，例如共定位，受激发射耗竭（STED）超分辨率成像，蛋白质组MS / MS分析和蛋白质印迹分析。该探针代表了线粒体膜结合的VDP荧光标记的罕见例子，可以为构建VDP特异性荧光探针提供新的方法，以更深入地了解其在线粒体相关疾病中的作用。

更新日期：2018-03-23

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