Abstract In this study, polyaniline functionalized graphene quantum dots composite with excellent performance was successfully prepared by situ polymerized, and it was applied to fabricate electrochemical sensor for analysis of calycosin in the complex samples of traditional Chinese medicine. The polyaniline functionalized graphene quantum dots composite was characterized by Fourier transform infrared spectroscopy and Raman analysis, to indicate that polyaniline functionalized graphene quantum dots composite was successfully synthesized. The morphology and microstructure of the modified electrodes were characterized by Scanning electron microscope and Transmission electron microscope. According to the results of cyclic voltammetry and electrochemical impedance spectroscope, the glassy carbon electrode modified with polyaniline functionalized graphene quantum dots composite significantly increased the electron transfer rate on the surface of sensor. Under the optimized conditions, the fabricated sensor was used for the analysis of calycosin in Radix Hedysari and Radix Astragali by differential pulse voltammetry, which displayed acceptable reproducibility, long-term stability, good selectivity and sensitivity. The proposed sensor exhibited a wide detection range (1.1 × 10−5–3.52 × 10−4 mol/L) with a detection limit of 9.8 × 10−6 mol/L (S/N = 3). This study provided a novel and convenient method for rapid evaluation of the quality of Radix Hedysari and Radix Astragali.

中文翻译：

---

一种通过聚苯胺功能化石墨烯量子点制备的电化学传感器分析毛蕊花苷的新方法

摘要 本研究通过原位聚合成功制备了性能优异的聚苯胺功能化石墨烯量子点复合材料，并将其应用于制备分析中药复样中毛蕊花素的电化学传感器。通过傅里叶变换红外光谱和拉曼分析对聚苯胺功能化石墨烯量子点复合材料进行表征，表明成功合成了聚苯胺功能化石墨烯量子点复合材料。通过扫描电镜和透射电镜对修饰电极的形貌和微观结构进行了表征。根据循环伏安法和电化学阻抗谱的结果，聚苯胺功能化石墨烯量子点复合材料修饰的玻碳电极显着提高了传感器表面的电子转移速率。在优化后的条件下，将制备的传感器用于差示脉冲伏安法分析白花蛇舌草和黄芪中毛蕊花素，其重现性好、长期稳定性好、选择性和灵敏度良好。所提出的传感器具有较宽的检测范围（1.1 × 10-5–3.52 × 10-4 mol/L），检测限为 9.8 × 10-6 mol/L（S/N = 3）。本研究为快速评价八角莲和黄芪的质量提供了一种新颖、方便的方法。所制备的传感器用于差示脉冲伏安法分析八角莲和黄芪中毛蕊花素，显示出可接受的重现性、长期稳定性、良好的选择性和灵敏度。所提出的传感器具有较宽的检测范围（1.1 × 10-5–3.52 × 10-4 mol/L），检测限为 9.8 × 10-6 mol/L（S/N = 3）。本研究为快速评价八角莲和黄芪的质量提供了一种新颖、方便的方法。所制备的传感器用于差示脉冲伏安法分析八角莲和黄芪中毛蕊花素，显示出可接受的重现性、长期稳定性、良好的选择性和灵敏度。所提出的传感器具有较宽的检测范围（1.1 × 10-5–3.52 × 10-4 mol/L），检测限为 9.8 × 10-6 mol/L（S/N = 3）。本研究为快速评价八角莲和黄芪的质量提供了一种新颖、方便的方法。