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One step growth of electro-assisted BSA functionalized screen-printed carbon interface with improved antifouling characteristics
Journal of Electroanalytical Chemistry ( IF 4.1 ) Pub Date : 2018-05-01 , DOI: 10.1016/j.jelechem.2018.03.047
Mian Hasnain Nawaz , Gaelle Catanante , Jean Louis Marty , Akhtar Hayat

Abstract The present study was aimed to investigate the direct electrooxidation of bovine serum albumin (BSA) without stabilizer and adsorbent to design antifouling screen printed carbon interface. Herein, a simple strategy revealed good immobilization support for biological molecules, better conducting transducer interface, and above all improved antifouling properties which are typically contradictory in one material. BSA was electro grafted onto the carbon surface of screen printed electrode in presence of NBu4BF4. Furthermore, to inhibit non-specific adsorption diethanolamine (DEA) was used as a blocking agent followed by adsorption studies. The comprehensive study of various BSA protein concentrations, pH buffer solutions and interaction of different analyte molecules such as DNA and toxin molecules was conducted for the adopted procedure as model analytes. The BSA grafting protocol was also validated by comparing the analyte adsorptions with simply drop casted modifications. The obtained results not only demonstrated the antifouling characteristics of the transducer surface but also proved the obtained transducer surface as a conductive immobilization support. Most of the medical devices face complications and failures due to nonspecific biofouling of microorganisms, cells and proteins. Towards imparting these devices with nonfouling capabilities, this stabilizer free SBA grafting may result a highly promising bio-surface for biomedical and engineering applications.

中文翻译:

具有改进防污特性的电辅助 BSA 功能化丝网印刷碳界面的一步生长

摘要 本研究旨在研究无稳定剂和吸附剂的牛血清白蛋白(BSA)的直接电氧化,以设计防污丝网印刷碳界面。在这里,一个简单的策略揭示了对生物分子的良好固定支持、更好的传导换能器界面,以及最重要的是改进的防污性能,这在一种材料中通常是矛盾的。在 NBu4BF4 存在下,BSA 被电接枝到丝网印刷电极的碳表面。此外,为了抑制非特异性吸附,使用二乙醇胺 (DEA) 作为封闭剂,然后进行吸附研究。各种BSA蛋白浓度的综合研究,pH 缓冲溶液和不同分析物分子(如 DNA 和毒素分子)的相互作用作为模型分析物用于采用的程序。通过将分析物吸附与简单滴铸修改进行比较,还验证了 BSA 接枝协议。所得结果不仅证明了换能器表面的防污特性,而且证明了所获得的换能器表面作为导电固定支撑。由于微生物、细胞和蛋白质的非特异性生物污染,大多数医疗设备面临并发症和故障。为了赋予这些设备防污能力,这种不含稳定剂的 SBA 接枝可能会为生物医学和工程应用带来非常有前途的生物表面。通过将分析物吸附与简单滴铸修改进行比较,还验证了 BSA 接枝协议。所得结果不仅证明了换能器表面的防污特性,而且证明了所获得的换能器表面作为导电固定支撑。由于微生物、细胞和蛋白质的非特异性生物污染,大多数医疗设备面临并发症和故障。为了赋予这些设备防污能力,这种不含稳定剂的 SBA 接枝可能会为生物医学和工程应用带来非常有前途的生物表面。通过将分析物吸附与简单滴铸修改进行比较,还验证了 BSA 接枝协议。所得结果不仅证明了换能器表面的防污特性,而且证明了所获得的换能器表面作为导电固定支撑。由于微生物、细胞和蛋白质的非特异性生物污染,大多数医疗设备面临并发症和故障。为了赋予这些设备防污能力,这种不含稳定剂的 SBA 接枝可能会为生物医学和工程应用带来非常有前途的生物表面。所得结果不仅证明了换能器表面的防污特性,而且证明了所获得的换能器表面作为导电固定支撑。由于微生物、细胞和蛋白质的非特异性生物污染,大多数医疗设备面临并发症和故障。为了赋予这些设备防污能力,这种不含稳定剂的 SBA 接枝可能会为生物医学和工程应用带来非常有前途的生物表面。所得结果不仅证明了换能器表面的防污特性,而且证明了所获得的换能器表面作为导电固定支撑。由于微生物、细胞和蛋白质的非特异性生物污染,大多数医疗设备面临并发症和故障。为了赋予这些设备防污能力,这种不含稳定剂的 SBA 接枝可能会为生物医学和工程应用带来非常有前途的生物表面。
更新日期:2018-05-01
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