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On the Correlation between the Microscopic Structure and Properties of Phosphate-Cross-Linked Chitosan Gels
ACS Applied Materials & Interfaces ( IF 8.3 ) Pub Date : 2018-03-23 00:00:00 , DOI: 10.1021/acsami.8b01834 Pasquale Sacco 1 , Francesco Brun 2 , Ivan Donati 1 , Davide Porrelli 3 , Sergio Paoletti 1 , Gianluca Turco 3
ACS Applied Materials & Interfaces ( IF 8.3 ) Pub Date : 2018-03-23 00:00:00 , DOI: 10.1021/acsami.8b01834 Pasquale Sacco 1 , Francesco Brun 2 , Ivan Donati 1 , Davide Porrelli 3 , Sergio Paoletti 1 , Gianluca Turco 3
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Ionic chitosan gels fabricated using multivalent anions, tripolyphosphate (TPP) or pyrophosphate (PPi), respectively, have been investigated as potential biomaterials to be used in tissue engineering. Starting from the hypothesis that the polymer mesh texture at the microscale affects the final performance of the resulting materials, an innovative image analysis approach is presented in the first part of the article, which is aimed at deriving quantitative information from transmission electron microscopy images. The image analysis of the (more extended) central area of the gel networks revealed differences between both the cross-linking densities and pore size distributions of the two systems, the TPP gels showing a higher connectivity. Chitosan–TPP gels showed a limited degradation in simulated physiological media up to 6 weeks, reasonably ascribed to the texture of the (more extended) central area of the gels, whereas PPi counterparts degraded almost immediately. The release profiles and the calculation of diffusion coefficients for bovine serum albumin and cytochrome c, herein used as model payloads, indicated a different release behavior depending on the polymer network homogeneity/inhomogeneity and molecular weight of loaded molecules. This finding was ascribed to the marked inhomogeneity of the PPi gels (at variance with the TPP ones), which had been demonstrated in our previous work. Finally, thorough in vitro studies demonstrated good biocompatibility of both chitosan gels, and because of this feature, they can be used as suitable scaffolds for cellular colonization and metabolic activity.
中文翻译：
磷酸酯交联壳聚糖凝胶的微观结构与性能的相关性
分别使用多价阴离子，三聚磷酸根（TPP）或焦磷酸根（PPi）制备的离子型壳聚糖凝胶已被研究为可用于组织工程的潜在生物材料。从微尺度上的聚合物网状结构会影响最终材料的最终性能这一假设出发，本文的第一部分提出了一种创新的图像分析方法，旨在从透射电子显微镜图像中获得定量信息。凝胶网络（扩展程度更大）的中心区域的图像分析显示了两个系统的交联密度和孔径分布之间的差异，TPP凝胶显示出更高的连通性。壳聚糖–TPP凝胶在长达6周的模拟生理介质中显示出有限的降解，合理地归因于凝胶（更延伸的）中心区域的质地，而PPi对应物几乎立即降解。牛血清白蛋白和细胞色素的释放曲线和扩散系数的计算本文用作模型有效载荷的c表示不同的释放行为，具体取决于聚合物网络的均质性/不均质性和负载分子的分子量。这一发现归因于PPi凝胶的显着不均匀性（与TPP凝胶不同），这在我们之前的工作中得到了证明。最后，彻底的体外研究表明两种壳聚糖凝胶均具有良好的生物相容性，并且由于此功能，它们可用作细胞定植和代谢活性的合适支架。
更新日期：2018-03-23
中文翻译：
磷酸酯交联壳聚糖凝胶的微观结构与性能的相关性
分别使用多价阴离子，三聚磷酸根（TPP）或焦磷酸根（PPi）制备的离子型壳聚糖凝胶已被研究为可用于组织工程的潜在生物材料。从微尺度上的聚合物网状结构会影响最终材料的最终性能这一假设出发，本文的第一部分提出了一种创新的图像分析方法，旨在从透射电子显微镜图像中获得定量信息。凝胶网络（扩展程度更大）的中心区域的图像分析显示了两个系统的交联密度和孔径分布之间的差异，TPP凝胶显示出更高的连通性。壳聚糖–TPP凝胶在长达6周的模拟生理介质中显示出有限的降解，合理地归因于凝胶（更延伸的）中心区域的质地，而PPi对应物几乎立即降解。牛血清白蛋白和细胞色素的释放曲线和扩散系数的计算本文用作模型有效载荷的c表示不同的释放行为，具体取决于聚合物网络的均质性/不均质性和负载分子的分子量。这一发现归因于PPi凝胶的显着不均匀性（与TPP凝胶不同），这在我们之前的工作中得到了证明。最后，彻底的体外研究表明两种壳聚糖凝胶均具有良好的生物相容性，并且由于此功能，它们可用作细胞定植和代谢活性的合适支架。
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