In the present paper, bioactive peptides from cauliflower by-products (leaves and stems) were investigated. Alcalase® protein hydrolysis time and pH conditions were optimized, then the hydrolysates were fractionated by preparative RP-HPLC into 12 fractions. Each fraction was tested for the ABTS (2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)), and DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity and for ACE (angiotensin-converting enzyme) inhibitor activity. The peptides in the most active fractions were identified by peptidomics technologies and screened for bioactivity by the use of bioinformatics. For ACE-inhibitor activity, two peptides were synthetized, APYDPDWYYIR and SKGFTSPLF, which provided an EC₅₀ value of 2.59 μmol L⁻¹ and 15.26 μmol L⁻¹, respectively. For the ABTS radical scavenging activity, SKGFTSPLF and LDDPVFRPL were tested, and provided an EC₅₀ of 10.35 μmol L⁻¹ and 8.29 μmol L⁻¹, respectively. For the DPPH radical scavenging activity, SKGFTSPLF and LRAPPGWTGR were tested and provided an EC₅₀ of 8.2 μmol L⁻¹ and 5.26 μmol L⁻¹, respectively.

在本文中，对花椰菜副产物（叶和茎）的生物活性肽进行了研究。优化了Alcalase®蛋白质的水解时间和pH条件，然后通过制备型RP-HPLC将水解产物分馏为12个馏分。测试了每个馏分的ABTS（2,2'-叠氮基双（3-乙基苯并噻唑啉-6-磺酸））和DPPH（2,2-二苯基-1-吡啶并肼基）自由基清除活性以及ACE（血管紧张素-转化酶）抑制剂的活性。通过肽组学技术鉴定活性最高的级分中的肽，并通过使用生物信息学筛选生物活性。对于ACE抑制剂活性，两种肽synthetized，APYDPDWYYIR和SKGFTSPLF，这提供了一种EC ₅₀的2.59微摩尔的L值^-1和15.26微摩尔大号^-1， 分别。对于ABTS自由基清除活性，SKGFTSPLF和LDDPVFRPL进行了测试，并提供了一种EC ₅₀ 10.35微摩尔L的^-1和8.29微摩尔大号^-1分别。对于DPPH自由基清除活性，SKGFTSPLF和LRAPPGWTGR进行测试，提供了一种EC ₅₀ 8.2微摩尔L的^-1和5.26微摩尔大号^-1分别。