The present study aims to investigate the constituents, antioxidant activities and hepatoprotective effect of polyphenols extracted from Coreopsis tinctoria buds (CTB-PE) against acute hepatic injury. HPLC analysis showed that six compounds were identified from polyphenolic extracts. The determination of antioxidant activities revealed that the CTB-PE had DPPH radical scavenging activity, ferric-reducing antioxidant power and oxygen radical absorbance capacity. The animal experiments verified that CTB-PE pretreatment ameliorated the D-galactosamine/lipopolysaccharide-induced alterations in histopathological changes, serum biochemical indicators, antioxidant capacity and lipid peroxidation. The potential mechanisms of the hepatoprotective effect might be that CTB-PE treatment significantly enhanced the protein and mRNA expression levels of NF-E2-related factor 2 (Nrf2), peroxisome proliferator-activated receptors α (PPARα) and γ (PPARγ). It suggested that the protective effect of CTB-PE against liver damage was likely attributed to eliminate free radicals, inhibit oxidative stress response and mediate inflammatory response.

本研究旨在研究从金鸡油中提取的多酚的成分，抗氧化活性和保肝作用。芽（CTB-PE）可以抵抗急性肝损伤。HPLC分析表明，从多酚提取物中鉴定出六种化合物。抗氧化剂活性的测定表明，CTB-PE具有DPPH自由基清除活性，三价铁还原抗氧化剂能力和氧自由基吸收能力。动物实验证明，CTB-PE预处理可改善D-半乳糖胺/脂多糖诱导的组织病理学变化，血清生化指标，抗氧化能力和脂质过氧化作用的变化。肝脏保护作用的潜在机制可能是CTB-PE处理显着提高了NF-E2相关因子2（Nrf2），过氧化物酶体增殖物激活受体α（PPARα）和γ（PPARγ）的蛋白质和mRNA表达水平。