In the present study we investigated the anti-inflammatory activity of Syzygium cumini seeds using lipopolysaccharide (LPS) stimulated RAW264.7 cells to reveal the underlying mechanism of action. Syzygium cumini seeds effectively reduced the production of nitric oxide (NO) and pro- inflammatory cytokines (IL-6, IL-10, INF-γ, IL-β, TNF-α). Syzygium cumini fractions also inhibited LPS-activated lipoxygenases and reduced the levels of iNOS and COX-2 mRNA expression in murine macrophages. These effects were mediated by impaired translocation of nuclear factor (NF)-κB and inhibition of the phosphorylation of JNK, ERK and p38 in LPS-stimulated RAW264.7 cells. This study gives scientific evidence that Syzygium cumini seeds inhibits the LPS-induced expression of inflammatory mediators via suppression of JNK, ERK and p38 mediated NF-κB pathway as well as down regulation of iNOS and COX-2.

在本研究中，我们使用脂多糖（LPS）刺激的RAW264.7细胞研究了孜孜孜然（Syzygium cumini）种子的抗炎活性，以揭示其潜在的作用机理。Syzygium cumini种子可有效减少一氧化氮（NO）和促炎细胞因子（IL-6，IL-10，INF-γ，IL-β，TNF-α）的产生。Syzygium cumini组分还抑制了小鼠巨噬细胞中LPS激活的脂加氧酶并降低了iNOS和COX-2 mRNA的表达水平。这些作用是由LPS刺激的RAW264.7细胞中核因子（NF）-κB的易位受损和JNK，ERK和p38磷酸化的抑制介导的。这项研究提供了科学证据，表明孜孜孜然 种子通过抑制JNK，ERK和p38介导的NF-κB通路以及下调iNOS和COX-2来抑制LPS诱导的炎症介质表达。