The microbiota from artisanal cheeses produced in the Amazonian region is evaluated. Samples of artisanal cheeses were obtained from markets in Conceição do Araguaia and Redenção (Pará, Brazil) over rainy and dry seasons, and their biodiversity was assessed by culture-dependent and culture-independent methods. Mean counts of lactic acid bacteria (LAB) in cheeses ranged from 7.32 to 8.84 log CFU/g, for both seasons. Members of genera Lactobacillus, Lactococcus, Weissella, Enterococcus, Pediococcus, and Leuconostoc were predominant. The amplification of the 16S rRNA V6-V9 region, followed by a temporal temperature-gradient gel electrophoresis (TTGE) and sequencing of the TTGE bands revealed important differences in the microbial composition variability between samples from the two seasons and among cheese samples analyzed. TTGE showed the presence of microorganisms that are frequently found in cheese, such as L. lactis subsp. lactis, as well as other non-usual species, such as Macrococcus caseolyticus and Corynebacterium variabile. Moreover, TTGE analysis revealed the presence of microorganisms that have been isolated from other types of foods (Paralactobacillus selangorenses) along with some not usually found in foods, such as Exiguobacterium acetylicum, plus the presence of pathogenic microorganisms (Granulicatella elegans and Aerococcus sanguinicola). The present molecular approaches combined with culture-dependent methods provided a more detailed description of the microbial ecology of traditional cheeses from the Amazonian region in northern Brazil.

对来自亚马逊地区生产的手工奶酪的微生物群进行了评估。在雨季和旱季，从阿拉斯加的Conceiçãodo Araguaia和Redenção（巴西帕拉）的市场中获得手工奶酪的样品，并通过与文化有关和与文化无关的方法对它们的生物多样性进行了评估。在两个季节中，奶酪中乳酸菌（LAB）的平均计数范围为7.32至8.84 log CFU / g。属的成员乳杆菌，乳酸乳球菌，魏斯氏，肠球菌属，片球菌和明串珠菌占主导地位。扩增16S rRNA V6-V9区，然后进行瞬时温度梯度凝胶电泳（TTGE）和TTGE条带测序，发现两个季节的样品之间和所分析的奶酪样品之间的微生物组成变异性存在重要差异。TTGE显示出存在于奶酪中的常见微生物，例如乳酸乳杆菌亚种。乳酸以及其他非常规物种，如干酪大球菌和变异棒状杆菌。此外，TTGE分析表明，已经从其他类型的食品中分离的微生物的存在（Paralactobacillus selangorenses）和一些通常在食品中不常见的食物，例如乙酰肠杆菌（Exiguobacterium aceticum），以及病原性微生物（秀丽线虫（Granulicatella elegans）和Aerococcus sanguinicola）。本分子方法与依赖文化的方法相结合，对巴西北部亚马逊地区传统奶酪的微生物生态学提供了更详细的描述。