Bisphenol A (BPA) is an organic compound from the phenolic group commonly used for the production of plastics. The use of BPA in food and drinking water containers carries a significant risk to human health since BPA can be washed out and enter consumables. BPA entering the human body with food shows a multi-directional effect and causes disorders in the functioning of many systems and organs. There is no current knowledge about the effects of BPA on the enteric nervous system. The purpose of the present study was to verify the influence of BPA on tolerable daily intake (TDI) dose (0.05 mg/kg body weight/day) and a dose ten times higher than TDI (0.5 mg/kg body weight/day) administered for 28 days on the porcine duodenum. The neurochemical characterization of the enteric neurons to five active neuronal substances was then investigated: substance P (SP), vasoactive intestinal polypeptide (VIP), galanin (GAL), vesicular acetylcholine transporter (VAChT) or cocaine- and amphetamine-regulated transcript peptide (CART) with double immunofluorescence method. Both doses of BPA caused visible changes in duodenal immunoreactivity to the majority of neuronal factors studied and the obtained results show that even TDI dose may affect the living organism.

双酚A（BPA）是通常用于生产塑料的酚类有机化合物。在食品和饮用水容器中使用BPA会对人体健康构成重大威胁，因为BPA可以洗净并进入消耗品。BPA与食物一起进入人体会表现出多方面的作用，并会导致许多系统和器官的功能紊乱。目前尚无关于BPA对肠神经系统影响的知识。本研究的目的是验证BPA对可耐受的每日摄入量（TDI）剂量（0.05 mg / kg体重/天）和比所施用的TDI（0.5 mg / kg体重/天）高十倍的剂量的影响在猪十二指肠上放置28天。然后研究了肠神经元对五种活性神经元物质的神经化学特性：物质P（SP），血管活性肠多肽（VIP），甘丙肽（GAL），水泡乙酰胆碱转运蛋白（VAChT）或可卡因和苯丙胺调节的转录肽（CART），具有双重免疫荧光方法。两种剂量的双酚A对大多数研究的神经元因子均引起十二指肠免疫反应性的可见变化，获得的结果表明，即使TDI剂量也可能影响活生物体。