Pig ear skin membrane-covered glucose biosensor based on oxygen electrode has been assessed as a tool to evaluate glucose penetration through skin in-vitro. For this, glucose oxidase (GOx) was immobilised on oxygen electrode and covered with the skin membrane. Exposing this electrode to the solution of glucose resulted in glucose penetration though skin membrane, its oxidation catalysed by GOx, consumption of O₂ and decrease of the current of the oxygen electrode. By processing the biosensor responses to glucose, we found that glucose penetration through 250 µm thick skin membrane is slow; 90% of steady-state current response was reached in 32( ± 22) min. Apparent diffusion coefficient for glucose in skin was found to be equal to 0.15( ± 0.07)* 10⁻⁶ cm² s⁻¹. This value is 45 times lower than glucose diffusion coefficient in water. Tape-stripping of stratum corneum (SC) allows considerably faster glucose penetration. The electrodes covered with tape-stripped skin reached 90% of steady-state current response in 5.0(± 2.7) min. The theoretical estimate of glucose flux through SC was considered exploiting four-pathway theory of transdermal penetration. Theoretical flux values were more that three orders lower than measured experimentally. This high discrepancy might indicate that glucose penetration through healthy human skin could be even slower, allowing much lower flux, than it was found in our study for skin membranes from pig ears.

基于氧电极的猪耳皮肤膜覆盖葡萄糖生物传感器已被评估为评估葡萄糖体外渗透皮肤的工具。为此，将葡萄糖氧化酶（GOx）固定在氧气电极上并覆盖了皮肤膜。将该电极暴露于葡萄糖溶液中会导致葡萄糖透过皮肤膜，其被GOx催化氧化，消耗O ₂并降低氧电极的电流。通过处理生物传感器对葡萄糖的反应，我们发现葡萄糖通过250 µm厚的皮肤膜的渗透速度很慢。在32（±22）分钟内达到90％的稳态电流响应。发现皮肤中葡萄糖的表观扩散系数等于0.15（±0.07）* 10 ^-6 cm ² s^-1。该值比水中的葡萄糖扩散系数低45倍。剥离角质层（SC）可以大大加快葡萄糖的渗透速度。用胶带剥开的皮肤覆盖的电极在5.0（±2.7）分钟内达到稳态电流响应的90％。通过透皮渗透的四途径理论考虑了通过SC的葡萄糖通量的理论估计。理论通量值比实验测量值低三个数量级。这种高度差异可能表明，通过健康的人类皮肤，葡萄糖的渗透速度甚至可能比我们从猪耳的皮肤膜的研究中发现的速度甚至更低，允许的通量要低得多。