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Analytical Method To Detect and Quantify Avocatin B in Hass Avocado Seed and Pulp Matter
Journal of Natural Products ( IF 3.3 ) Pub Date : 2018-03-22 00:00:00 , DOI: 10.1021/acs.jnatprod.7b00914 Nawaz Ahmed 1 , Richard W. Smith 2 , Juan J. Aristizabal Henao 3 , Ken D. Stark 3 , Paul A. Spagnuolo 1
Journal of Natural Products ( IF 3.3 ) Pub Date : 2018-03-22 00:00:00 , DOI: 10.1021/acs.jnatprod.7b00914 Nawaz Ahmed 1 , Richard W. Smith 2 , Juan J. Aristizabal Henao 3 , Ken D. Stark 3 , Paul A. Spagnuolo 1
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Avocatin B, an avocado-derived compound mixture, was demonstrated recently to possess potent anticancer activity by selectively targeting and eliminating leukemia stem cells. Avocatin B is a mixture of avocadene and avocadyne, two 17-carbon polyhydroxylated fatty alcohols (PFAs), first discovered in avocado seeds; their quantities in avocado pulp are unknown. Analytical methods to detect avocado seed PFAs have utilized NMR spectroscopy and GC-MS; both of these lack quantitative capacity and accuracy. Herein, we report a sensitive LC-MS method for the quantitation of avocadene and avocadyne in avocado seed and pulp. The method has a reliable and linear response range of 0.1–50 μM (0.03–17.2 ng/μL) for both avocadene and avocadyne (r2 > 0.990) with a lower limit of quantitation (LLOQ) of 0.1 μM. The intra- and interassay accuracy and precision of the quality control (QC) samples at LLOQ showed ≤18.2% percentage error and ≤14.4% coefficient of variation (CV). The intra- and interassay accuracy and precision for QC samples at low and high concentrations were well below 10% error and CV. This method was successfully applied to quantify avocadene and avocadyne in total lipid extracts of Hass avocado pulp and seed matter.
中文翻译：
检测和定量哈斯鳄梨种子和果肉物质中鳄梨B的分析方法
最近证实，鳄梨衍生的混合物Avocatin B具有有效的抗癌活性，它可以选择性地靶向并消除白血病干细胞。Avocatin B是Avocadene和Avocadyne的混合物，这是两种17碳多羟基脂肪醇（PFA），最早在鳄梨种子中发现；它们在鳄梨果肉中的含量是未知的。用于检测鳄梨种子中PFA的分析方法已经利用NMR光谱和GC-MS进行了分析。这些都缺乏定量能力和准确性。在这里，我们报告了一种灵敏的LC-MS方法，用于定量鳄梨种子和果肉中的阿伏丁香精油和阿瓦丁炔。该方法对avocadene和avocadyne的线性响应范围均为0.1–50μM（0.03–17.2 ng /μL）（r 2> 0.990），定量下限（LLOQ）为0.1μM。LLOQ的质量控制（QC）样品的批内和批间准确性和精密度显示，≤18.2％的百分比误差和≤14.4％的变异系数（CV）。低和高浓度QC样品的批内和批间准确性和精密度均远低于10％误差和CV。该方法已成功用于量化哈斯鳄梨果肉和种子中总脂质提取物中的阿伏丁香精和阿瓦丁炔。
更新日期：2018-03-22
中文翻译：
检测和定量哈斯鳄梨种子和果肉物质中鳄梨B的分析方法
最近证实，鳄梨衍生的混合物Avocatin B具有有效的抗癌活性，它可以选择性地靶向并消除白血病干细胞。Avocatin B是Avocadene和Avocadyne的混合物，这是两种17碳多羟基脂肪醇（PFA），最早在鳄梨种子中发现；它们在鳄梨果肉中的含量是未知的。用于检测鳄梨种子中PFA的分析方法已经利用NMR光谱和GC-MS进行了分析。这些都缺乏定量能力和准确性。在这里，我们报告了一种灵敏的LC-MS方法，用于定量鳄梨种子和果肉中的阿伏丁香精油和阿瓦丁炔。该方法对avocadene和avocadyne的线性响应范围均为0.1–50μM（0.03–17.2 ng /μL）（r 2> 0.990），定量下限（LLOQ）为0.1μM。LLOQ的质量控制（QC）样品的批内和批间准确性和精密度显示，≤18.2％的百分比误差和≤14.4％的变异系数（CV）。低和高浓度QC样品的批内和批间准确性和精密度均远低于10％误差和CV。该方法已成功用于量化哈斯鳄梨果肉和种子中总脂质提取物中的阿伏丁香精和阿瓦丁炔。
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